Isolation of a new L-amino acid oxidase from Crotalus durissus cascavella venom

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorToyama, M. H.
dc.creatorToyama, D. D.
dc.creatorPassero, LFD
dc.creatorLaurenti, M. D.
dc.creatorCorbett, C. E.
dc.creatorTomokane, T. Y.
dc.creatorFonseca, F. V.
dc.creatorAntunes, E.
dc.creatorJoazeiro, P. P.
dc.creatorBeriam, LOS
dc.creatorMartins, MAC
dc.creatorMonteiro, HSA
dc.creatorFonteles, M. C.
dc.date2014-05-20T15:25:50Z
dc.date2016-10-25T18:00:25Z
dc.date2014-05-20T15:25:50Z
dc.date2016-10-25T18:00:25Z
dc.date2006-01-01
dc.date.accessioned2017-04-05T23:54:23Z
dc.date.available2017-04-05T23:54:23Z
dc.identifierToxicon. Oxford: Pergamon-Elsevier B.V., v. 47, n. 1, p. 47-57, 2006.
dc.identifier0041-0101
dc.identifierhttp://hdl.handle.net/11449/36176
dc.identifierhttp://acervodigital.unesp.br/handle/11449/36176
dc.identifier10.1016/j.toxicon.2005.09.008
dc.identifierWOS:000235381200006
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2005.09.008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/879745
dc.descriptionA novel L-amino acid oxidase (LAO) (Casca LAO) from Crotalus durissus cascavella venom was purified to a high degree of molecular homogeneity using a combination of molecular exclusion and ion-exchange chromatography system. The purified monomer of LAO presented a molecular mass of 68 kDa and pI estimated in 5.43, which were determined by two-dimensional electrophoresis. The 71st N-terminal amino acid sequence of the LAO from Crotalus durissus cascavella presented a high amino acid sequence similarities with other LAOs from Colloselasma rhosostoma, Crotalus adamanteus, Agkistrodon h. blomhoffi, Agkistrodon h. halys and Trimeresurus stejnegeri. LAO displayed a Michaelis-Menten behavior with a kilometer of 46.7 mu M and an optimum pH for enzymatic activity of 6.5. Casca LAO induced a dose-dependent platelet aggregation, which was abolished by catalase and inhibited by indomethacin and aspirin. These results suggest that the production of H2O2 is involved in subsequent activation of inflammatory enzymes, such as thromboxane. Casca LAO also inhibited the bacterial Growth of Gram-negative (Xanthomonas axonopodis pv passiflorae) and Gram-positive (S. mutans) strains. Electron microscopy assessments of both bacterial strains suggest that the hydrogen peroxide produced by LAO induce bacterial membrane rupture and consequently loss of cytoplasmatic content. This LAO exhibited a high antileishmanic activity against the promastigote of Leishmania amazonensis in vitro, its activity was dependent on the production of hydrogen peroxide, and the 50% inhibitory concentration was estimated in 2.39 mu g/ml. (C) 2005 Elsevier Ltd. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationToxicon
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCrotalus durissus cascavella
dc.subjectantibacterial
dc.subjectantimicrobial
dc.subjecthydrogen peroxide
dc.subjectgyroxin
dc.subjectleishmanicidal
dc.titleIsolation of a new L-amino acid oxidase from Crotalus durissus cascavella venom
dc.typeOtro


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