Comparison of hexamethyidisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludge

Araujo, J. C.; Teran, F. C.; Oliveira, R. A.; Nour, EAA; Montenegro, MAP; Campos, JR; Vazoller, R. F.

Otro

Registro en:

Journal of Electron Microscopy. Oxford: Oxford Univ Press, v. 52, n. 4, p. 429-433, 2003.

0022-0744

http://hdl.handle.net/11449/35237

http://acervodigital.unesp.br/handle/11449/35237

10.1093/jmicro/52.4.429

WOS:000185860700011

http://dx.doi.org/10.1093/jmicro/52.4.429

http://repositorioslatinoamericanos.uchile.cl/handle/2250/879009

Autor

Araujo, J. C.

Teran, F. C.

Oliveira, R. A.

Nour, EAA

Montenegro, MAP

Campos, JR

Vazoller, R. F.

Institución

Universidade Paulista Júlio de Mesquita Filho (Brasil)

Resumen

We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.

Materias

anaerobic biofilms

critical point drying method

granular sludge

HMDS treatment

SEM analysis

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