Simultaneous observation of collagen and elastin in normal and pathological tissues: analysis of Sirius-red-stained sections by fluorescence microscopy

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBorges, L. F.
dc.creatorTaboga, SR
dc.creatorGutierrez, P. S.
dc.date2014-05-20T15:24:25Z
dc.date2016-10-25T17:58:38Z
dc.date2014-05-20T15:24:25Z
dc.date2016-10-25T17:58:38Z
dc.date2005-06-01
dc.date.accessioned2017-04-05T23:47:13Z
dc.date.available2017-04-05T23:47:13Z
dc.identifierCell and Tissue Research. New York: Springer, v. 320, n. 3, p. 551-552, 2005.
dc.identifier0302-766X
dc.identifierhttp://hdl.handle.net/11449/35035
dc.identifierhttp://acervodigital.unesp.br/handle/11449/35035
dc.identifier10.1007/s00441-005-1108-6
dc.identifierWOS:000229511300020
dc.identifier0000-0002-0970-4288
dc.identifierhttp://dx.doi.org/10.1007/s00441-005-1108-6
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/878845
dc.descriptionIn order to observe collagen and elastic fibers simultaneously, sections of human aorta, skin, lung, liver, and bladder were stained by Sirius red and analyzed by fluorescence microscopy. In all cases, the fibers of collagen presented the characteristic fluorescent red-orange color that results from the interaction of this extracellular protein with the dye, whereas elastic fibers showed strong green fluorescence (intrinsic fluorescence). This method efficiently detects collagen and elastic fibers when these two structures are present and could have valuable applications in processes that involves both fibers.
dc.languageeng
dc.publisherSpringer
dc.relationCell and Tissue Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcollagen
dc.subjectelastin
dc.subjectSirius red
dc.subjectfluorescence
dc.subjecthuman
dc.titleSimultaneous observation of collagen and elastin in normal and pathological tissues: analysis of Sirius-red-stained sections by fluorescence microscopy
dc.typeOtro


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