Sterilization of immature blowflies (Calliphoridae) for use in larval therapy

dc.contributorUniversidade Federal de Pelotas (UFPEL)
dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorThyssen, Patrícia Jaqueline
dc.creatorNassau, Mariana Prado
dc.creatorNitsche, Maria José Trevizani [UNESP]
dc.creatorLeite, Domingos da Silva
dc.date2016-04-01T18:43:15Z
dc.date2016-04-01T18:43:15Z
dc.date2013
dc.date.accessioned2023-09-12T09:10:41Z
dc.date.available2023-09-12T09:10:41Z
dc.identifierhttp://www.interesjournals.org/JMMS
dc.identifierJournal of Medicine and Medical Sciences, v. 4, n. 10, p. 405-409, 2013.
dc.identifier2141-9477
dc.identifierhttp://hdl.handle.net/11449/136911
dc.identifierISSN2141-9477-2013-04-10-405-409.pdf
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8786153
dc.descriptionThe larval therapy involves the intentional application of sterilized and alive dipterans larvae on nonhealing wounds with the purpose of cleaning out the necrotic tissue to facilitate the healing process. This study aimed to evaluate the sterilization process and survival of Chrysomya megacephala (Fabricius), Chrysomya putoria (Wiedemann) and Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae) larvae, after treatment with sodium hypochlorite (NaClO). The experimental groups for each species were: control group (CG), in which it was used water; 0.5% sodium hypochlorite group (0.5% SHG); and 1% SHG. For testing the time for each treatment, the eggs were washed for one or three minutes in the described solution. After, aliquots from the "rinsing water" were inoculated in Plate Count Agar (PCA) and blood agar (BA), with incubation at 37ºC and readings performed after 24 and 48 hours for both. For all species, there were no bacterial growth on PCA and BA regarding to both treatments, 0.5% SHG and 1.0% SHG. The survival rate and intervals of immersion were similar among the CG and the different concentrations of NaClO for all species. In conclusion, sterilization of blowflies' eggs made by NaClO is an efficient method to obtain the sterilized larvae for larval therapy.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Enfermagem, Botucatu, SP, Brasil
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Enfermagem, Botucatu, SP, Brasil
dc.descriptionFAPESP: 2012/06033-5
dc.format405-409
dc.languageeng
dc.relationJournal of Medicine and Medical Sciences
dc.rightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectDiptera
dc.subjectWounds
dc.subjectTreatment
dc.subjectMaggots
dc.subjectBiotherapy
dc.titleSterilization of immature blowflies (Calliphoridae) for use in larval therapy
dc.typeArtigo


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