Expression of α6 integrin subunit in bovine oocyte and its potential role during fertilization

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorGonçalves, Roseli Fernandes
dc.creatorBertolla, Ricardo Pimenta
dc.creatorOrlandi, Cássia Maria Barroso [UNESP]
dc.creatorBarnabe, Valquiria Hyppolito
dc.date2016-01-28T16:57:08Z
dc.date2016-01-28T16:57:08Z
dc.date2013
dc.date.accessioned2023-09-12T08:19:05Z
dc.date.available2023-09-12T08:19:05Z
dc.identifierhttp://www.scirp.org/journal/abb/
dc.identifierAdvances in Biociences and Biotechnology, v. 4, p. 617-622, 2013.
dc.identifier2156-8456
dc.identifierhttp://hdl.handle.net/11449/133914
dc.identifier10.4236/abb.2013.45081
dc.identifierISSN2156-8456-2013-04-617-622.pdf
dc.identifier3278725857184504
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8783164
dc.descriptionFertilization in mammals requires the successful completion of a sequence of steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion to produce a zygote. Although some integrin subunits are known to be associated with the plasma membrane of some mammalian oocytes and spermatozoa, the presence of α6 integrin on bovine oocytes with intact zona pellucida has not been reported. The present study was undertaken to evaluate the expression of α6 integrin subunit in bovine oocyte and to determine if in vitro binding to the zona pellucida and fertilization were affected by treating oocytes with α6 integrin subunit antibody. The α6 integrin subunit was identified on the bovine oocyte by immunocytochemistry. In vitro fertilization was significantly decreased when in vitro matured bovine oocytes were pre-incubated with α6 integrin subunit antibody at concentration 5 and 20 μg/mL, and spermoocyte binding increased. These studies demonstrated the presence of α6 integrin subunit on bovine oocyte, and its importance in fertilization and polyspermy.
dc.descriptionDepartment of Animal Reproduction, College of Veterinary Medicine and Animal Science, São Paulo University, São Paulo, Brazil
dc.descriptionDepartment of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil
dc.descriptionLaboratory of Animal Endocrinology, College of Veterinary Medicine, São Paulo State University, Araçatuba, Brazil
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Laboratory of Animal Endocrinology, College of Veterinary Medicine, Araçatuba, Brazil
dc.format617-622
dc.languageeng
dc.publisherScientific Research
dc.relationAdvances in Biociences and Biotechnology
dc.rightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectIntegrins
dc.subjectBovine oocytes
dc.subjectFertilization
dc.subjectSperm-oocyte binding
dc.titleExpression of α6 integrin subunit in bovine oocyte and its potential role during fertilization
dc.typeArtigo


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