Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Estadual de Londrina (UEL)
dc.creatorBianchi, Jaqueline [UNESP]
dc.creatorMantovani, Mario Sérgio
dc.creatorMarin-Morales, Maria Aparecida [UNESP]
dc.date2015-12-07T15:38:50Z
dc.date2015-12-07T15:38:50Z
dc.date2015-10-01
dc.date.accessioned2023-09-12T07:38:16Z
dc.date.available2023-09-12T07:38:16Z
dc.identifierhttp://dx.doi.org/10.1016/j.jes.2015.03.034
dc.identifierJournal Of Environmental Sciences (china), v. 36, p. 102-111, 2015.
dc.identifier1001-0742
dc.identifierhttp://hdl.handle.net/11449/131620
dc.identifier10.1016/j.jes.2015.03.034
dc.identifier26456612
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780899
dc.descriptionBased on the concentration of Malathion used in the field, we evaluated the genotoxic potential of low concentrations of this insecticide on meristematic and F1 cells of Allium cepa and on rat hepatoma tissue culture (HTC cells). In the A. cepa, chromosomal aberrations (CAs), micronuclei (MN), and mitotic index (MI) were evaluated by exposing the cells at 1.5, 0.75, 0.37, and 0.18mg/mL of Malathion for 24 and 48hr of exposure and 48hr of recovery time. The results showed that all concentrations were genotoxic to A. cepa cells. However, the analysis of the MI has showed non-relevant effects. Chromosomal bridges were the CA more frequently induced, indicating the clastogenic action of Malathion. After the recovery period, the higher concentrations continued to induce genotoxic effects, unlike the observed for the lowest concentrations tested. In HTC cells, the genotoxicity of Malathion was evaluated by the MN test and the comet assay by exposing the cells at 0.09, 0.009, and 0.0009mg/5mL culture medium, for 24hr of exposure. In the comet assay, all the concentrations induced genotoxicity in the HTC cells. In the MN test, no significant induction of MN was observed. The genotoxicity induced by the low concentrations of Malathion presented in this work highlights the importance of studying the effects of low concentrations of this pesticide and demonstrates the efficiency of these two test systems for the detection of genetic damage promoted by Malathion.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionDepartment of Biology, Institute of Biosciences, São Paulo State University (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, Brazil.
dc.descriptionDepartment of General Biology, Biological Science Centre, Univ Estadual de Londrina, 86061990, Londrina, PR, 6001, Brazil.
dc.descriptionDepartment of Biology, Institute of Biosciences, São Paulo State University (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, Brazil.
dc.descriptionDepartment of Biology, Institute of Biosciences, São Paulo State University (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, Brazil.
dc.descriptionDepartment of Biology, Institute of Biosciences, São Paulo State University (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, Brazil.
dc.descriptionFAPESP: 2005/57857-4
dc.format102-111
dc.languageeng
dc.publisherElsevier B. V.
dc.relationJournal Of Environmental Sciences (china)
dc.relation3.120
dc.relation0,879
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectChromosomal aberration
dc.subjectComet assay
dc.subjectHtc
dc.subjectMicronuclei
dc.subjectPesticide
dc.titleAnalysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture
dc.typeArtigo


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