Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: evidence of a broad arsenal of toxins

dc.contributorInstituto de Pesquisas Energéticas e Nucleares (IPEN)
dc.contributorUniversitätsklinikum Hamburg-Eppendorf
dc.contributorUniversität Hamburg
dc.contributorInstituto Butantan
dc.creatorViala, Vincent Louis
dc.creatorHildebrand, Diana
dc.creatorFucase, Tamara Mieco
dc.creatorSciani, Juliana Mozer
dc.creatorPrezotto-Neto, José Pedro
dc.creatorRiedner, Maria
dc.creatorSanches, Leonardo
dc.creatorNishimura, Paula Juliana
dc.creatorOguiura, Nancy
dc.creatorPimenta, Daniel Carvalho
dc.creatorSchlüter, Hartmut
dc.creatorBetzel, Christian
dc.creatorArni, Raghuvir Krishnaswami [UNESP]
dc.creatorSpencer, Patrick Jack
dc.date2015-12-07T15:38:29Z
dc.date2015-12-07T15:38:29Z
dc.date2015-09-25
dc.date.accessioned2023-09-12T07:38:01Z
dc.date.available2023-09-12T07:38:01Z
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2015.09.023
dc.identifierToxicon : Official Journal Of The International Society On Toxinology, 2015.
dc.identifier1879-3150
dc.identifierhttp://hdl.handle.net/11449/131605
dc.identifier10.1016/j.toxicon.2015.09.023
dc.identifier9162508978945887
dc.identifier26403864
dc.identifier0000-0003-2460-1145
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780884
dc.descriptionThe investigation of venoms has many clinical, pharmacological, ecological and evolutionary outcomes. The Crotalus spp. venom can cause hemorrhage, neurotoxicity, myotoxicity, coagulopathy and hypotension. Although neurotoxicity and hemorrhage usually does not occur for the same species, the rare Venezuelan species Crotalus vegrandis presents both characteristic. Different from the other species it has a restricted ecological niche and geographical distribution. Nevertheless, it has a raising medical importance as this rattlesnake population is increasing. Few works describe its neurotoxic and hemorrhagic features, but other toxins might play an important role in envenomation. We combined proteomic methods to identify for the first time the main components of it venom: 2D SDS-PAGE and gel-filtration chromatography for protein mixture decomplexation; LC-MS(2) of low molecular mass fractions and tryptic peptides; bioinformatic identification of toxin families and specific protein species based on unique peptide analysis and sequence database enriched with species-specific venom gland transcripts; and finally polyclonal anti-crotamine Western-blotting. Our results point to a broad arsenal of toxins in C. vegrandis venom: PIII and PII metalloproteases, crotoxin subunits, other phospholipases, isoforms of serine proteases and lectins, l-amino-acid oxidase, nerve growth factor, as well as other less abundant toxins.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenadação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionInstituto de Pesquisas Energéticas e Nucleares, Centro de Biotecnologia
dc.descriptionUniversitätsklinikum Hamburg-Eppendorf, Institut für Klinische Chemie
dc.descriptionUniversität Hamburg, Institut für Biochemie und Molekularbiologie
dc.descriptionInstituto Butantan, Laboratório Especial de Ecologia e Evolução
dc.descriptionUniversität Hamburg, Institut für Organische Chemie, Massenspektrometrie
dc.descriptionUniversität Hamburg, Institut für Biochemie und Molekularbiologie
dc.descriptionUniversidade Estadual Paulista, Departamento de Física, Instituto de Biociências, Letras e Ciências Exatas de São José do Rio Preto
dc.descriptionFAPESP: 2010/08585-8
dc.descriptionFAPESP: 2009/10305-8
dc.descriptionCAPES: 364/11
dc.languageeng
dc.publisherElsevier B. V.
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectCrotalus vegrandis
dc.subjectPeptide
dc.subjectProteomics
dc.subjectToxin
dc.subjectUracoan rattlesnake
dc.subjectVenom
dc.titleProteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: evidence of a broad arsenal of toxins
dc.typeArtigo


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