Improvement of an enzyme immunosorbent assay for detecting antibodies against Dioctophyma renale

dc.contributorUniversidade do Contestado (UnC)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorPedrassani, Daniela
dc.creatorNascimento, Adjair Antonio do [UNESP]
dc.creatorAndré, Marcos Rogério [UNESP]
dc.creatorMachado, Rosangela Zacarias [UNESP]
dc.date2015-12-07T15:38:03Z
dc.date2015-12-07T15:38:03Z
dc.date2015-09-15
dc.date.accessioned2023-09-12T07:37:37Z
dc.date.available2023-09-12T07:37:37Z
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2015.08.007
dc.identifierVeterinary Parasitology, v. 212, n. 3-4, p. 435-438, 2015.
dc.identifier1873-2550
dc.identifierhttp://hdl.handle.net/11449/131582
dc.identifier10.1016/j.vetpar.2015.08.007
dc.identifier3254990612451836
dc.identifier9139899895580513
dc.identifier26299933
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780861
dc.descriptionAn available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-Dioctophyma renale antibodies in the sera of dogs using, detection of parasite eggs in urine sediment as a reference test. ELISA uses a soluble antigenic preparation of esophagus of D. renale and the optimal dilutions of the antigen, serum and conjugate were determined by means of checker board titration, using positive (n=13) and negative (n=27) reference serum. The specificity and sensitivity of the ELISA were 93.8% and 92.3% respectively and the kappa index was good (0.76). These results suggest that ELISA described may prove to be an effective serological test for detecting dogs infected and exposed to this parasite mainly dogs that are not eliminating parasite eggs through their urine.
dc.descriptionDepartment of Veterinary Medicine, Universidade do Contestado (UnC), Rua Roberto Ehlke 86, CEP 89460-000 Canoinhas, SC, Brazil
dc.descriptionDepartment of Preventive Veterinary Medicine and Animal Reproduction, School of Agrarian and Veterinary Sciences (FCAV), Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900, Jaboticabal, SP, Brazil
dc.descriptionDepartment of Veterinary Pathology, FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900 Jaboticabal, SP, Brazil.
dc.descriptionDepartment of Preventive Veterinary Medicine and Animal Reproduction, School of Agrarian and Veterinary Sciences (FCAV), Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900, Jaboticabal, SP, Brazil
dc.descriptionDepartment of Veterinary Pathology, FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900 Jaboticabal, SP, Brazil.
dc.format435-438
dc.languageeng
dc.publisherElsevier B. V.
dc.relationVeterinary Parasitology
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectDioctophyma
dc.subjectDogs
dc.subjectElisa test
dc.subjectSerological diagnosis
dc.titleImprovement of an enzyme immunosorbent assay for detecting antibodies against Dioctophyma renale
dc.typeArtigo


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