Effect of avocado/soybean unsaponifiables on ligature-induced bone loss and bone repair after ligature removal in rats

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorAarhus University
dc.contributorMalmö University
dc.creatorOliveira, G. J. P. L. [UNESP]
dc.creatorPaula, L. G. F. [UNESP]
dc.creatorSouza, J. A. C. [UNESP]
dc.creatorSpin-Neto, R.
dc.creatorStavropoulos, A.
dc.creatorMarcantonio, R. A. C. [UNESP]
dc.date2015-12-07T15:36:48Z
dc.date2015-12-07T15:36:48Z
dc.date2015-08-06
dc.date.accessioned2023-09-12T07:36:29Z
dc.date.available2023-09-12T07:36:29Z
dc.identifierhttp://dx.doi.org/10.1111/jre.12312
dc.identifierJournal Of Periodontal Research, p. 1-10, 2015.
dc.identifier1600-0765
dc.identifierhttp://hdl.handle.net/11449/131516
dc.identifier10.1111/jre.12312
dc.identifier3534044399884035
dc.identifier26248485
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780795
dc.descriptionThe aim of this study was to evaluate the effects of administration of avocado/soybean unsaponifiable (ASU), a drug that is commonly used in the treatment of rheumatoid arthritis, on ligature-induced bone loss and bone repair after ligature removal in rats. Eighty-four rats were randomly assigned to four groups of equal size and received a daily gavage of either sterile saline [control (CTR)] or ASU (0.6 mg/kg), starting 7 d before (ASU/-7), on the day of (ASU/0) or 7 d after (ASU/+7) periodontitis induction. Periodontitis was induced by placing silk ligatures into the gingival sulcus of the second maxillary molars for 7 d; after 7 d, the ligatures were removed. Seven rats from each group were sacrificed, 7, 15 or 30 d after ligature removal. Bone resorption was evaluated by histomorphometry and micro-computed tomography (micro-CT). Immunohistochemistry was used to evaluate the expression of TRAP, RANKL and alkaline phosphatase (ALP), and quantitative PCR (qPCR) was used to evaluate the levels of interleukin-1beta (Il1β), tumor necrosis factor alpha (Tnfα), interleukin-6 (Il-6), Rankl and Alp. Statistical analysis was performed using the Shapiro-Wilk test, ANOVA and Tukey's test for normal data, and using the Kruskall-Wallis and Dunnet's tests for non-normal data (p < 0.05). Histomorphometry and micro-CT analysis showed greater bone resorption in the CTR group than in the ASU/0 (15 d) and ASU/+7 (7 and 15 d) groups. The CTR group also presented with a higher expression of TRAP (15 and 30 d) and RANKL (7 and 15 d) compared with ASU/0 and ASU/+7 groups. Similarly, qPCR analysis showed higher levels of Rankl and Il1β mRNAs, and lower levels of Alp mRNA, in the CTR group compared with all other groups (for all periods). ASU exhibited a positive effect on bone repair following ligature-induced periodontitis in rats.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionSection of Periodontology, Department of Diagnosis and Surgery, Araraquara School of Dentistry, University Estadual Paulista - UNESP, Araraquara, SP, Brazil.
dc.descriptionSection of Oral Radiology, Department of Dentistry, Aarhus University, Aarhus, Denmark.
dc.descriptionDepartment of Periodontology, Faculty of Odontology, Malmö University, Malmö, Sweden.
dc.descriptionSection of Periodontology, Department of Diagnosis and Surgery, Araraquara School of Dentistry, University Estadual Paulista - UNESP, Araraquara, SP, Brazil.
dc.descriptionFAPESP: 2010/13029-9
dc.descriptionFAPESP: 2009/17395-2
dc.format1-10
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationJournal Of Periodontal Research
dc.relation0,927
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectAnimal studies
dc.subjectAvocado/soybean unsaponifiables
dc.subjectHost modulation
dc.subjectPeriodontitis
dc.titleEffect of avocado/soybean unsaponifiables on ligature-induced bone loss and bone repair after ligature removal in rats
dc.typeArtigo


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