Comparative effects of fipronil and its metabolites sulfone and desulfinyl on the isolated rat liver mitochondria

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorTavares, Marco A. [UNESP]
dc.creatorPalma, Ivo D. F. [UNESP]
dc.creatorMedeiros, Hyllana C. D. [UNESP]
dc.creatorGuelfi, Marieli [UNESP]
dc.creatorSantana, Andréia T. [UNESP]
dc.creatorMingatto, Fábio E. [UNESP]
dc.date2015-12-07T15:35:59Z
dc.date2015-12-07T15:35:59Z
dc.date2015
dc.date.accessioned2023-09-12T07:35:36Z
dc.date.available2023-09-12T07:35:36Z
dc.identifierhttp://dx.doi.org/10.1016/j.etap.2015.06.013
dc.identifierEnvironmental Toxicology And Pharmacology, v. 40, n. 1, p. 206-214, 2015.
dc.identifier1872-7077
dc.identifierhttp://hdl.handle.net/11449/131467
dc.identifier10.1016/j.etap.2015.06.013
dc.identifier26143514
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780747
dc.descriptionFipronil is an insecticide extensively used to control pests in crops and animals. There are relates of poisoning due to exposure of fipronil in mammals and the liver has been suggested as potential target. In this study, we evaluated the effects of fipronil and its metabolites sulfone and desulfinyl on the bioenergetics, reactive oxygen species (ROS) production and calcium efflux from mitochondria isolated from rat liver. Fipronil (5-25 μM) inhibited state-3 respiration in mitochondria energized with glutamate plus malate, substrates of complex I of the respiratory chain and decreased the mitochondrial membrane potential resulting in inhibition of ATP synthesis. Fipronil also caused uncoupling in succinate-energized mitochondria and calcium efflux. The metabolites sulfone and desulfinyl also acted as mitochondrial inhibitors and uncouplers and caused calcium efflux, but with different potencies, being the sulfone the more potent one. These effects of fipronil and its metabolites on mitochondrial bioenergetics and calcium homeostasis may be related to toxic effects of the insecticide in the liver.
dc.descriptionLaboratory of Metabolic and Toxicological Biochemistry (LaBMeT), UNESP - Univ Estadual Paulista, Campus of Dracena, Dracena, SP, Brazil.
dc.descriptionLaboratory of Metabolic and Toxicological Biochemistry (LaBMeT), UNESP - Univ Estadual Paulista, Campus of Dracena, Dracena, SP, Brazil. Electronic address: fmingatto@dracena.unesp.br.
dc.descriptionLaboratory of Metabolic and Toxicological Biochemistry (LaBMeT), UNESP - Univ Estadual Paulista, Campus of Dracena, Dracena, SP, Brazil.
dc.descriptionLaboratory of Metabolic and Toxicological Biochemistry (LaBMeT), UNESP - Univ Estadual Paulista, Campus of Dracena, Dracena, SP, Brazil. Electronic address: fmingatto@dracena.unesp.br.
dc.format206-214
dc.languageeng
dc.publisherElsevier B. V.
dc.relationEnvironmental Toxicology And Pharmacology
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectFipronil
dc.subjectLiver
dc.subjectMetabolites
dc.subjectMitochondria
dc.subjectOxidative phosphorylation
dc.subjectToxicity
dc.titleComparative effects of fipronil and its metabolites sulfone and desulfinyl on the isolated rat liver mitochondria
dc.typeArtigo


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