Peptidome profiling of venom from the social wasp Polybia paulista

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorDias, Nathalia Batista [UNESP]
dc.creatorSouza, Bibiana Monson de [UNESP]
dc.creatorGomes, Paulo Cesar [UNESP]
dc.creatorBrigatte, Patricia [UNESP]
dc.creatorPalma, Mario Sergio [UNESP]
dc.date2015-12-07T15:33:07Z
dc.date2015-12-07T15:33:07Z
dc.date2015
dc.date.accessioned2023-09-12T07:32:02Z
dc.date.available2023-09-12T07:32:02Z
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2015.08.013
dc.identifierToxicon : Official Journal Of The International Society On Toxinology, 2015.
dc.identifier1879-3150
dc.identifierhttp://hdl.handle.net/11449/131256
dc.identifier10.1016/j.toxicon.2015.08.013
dc.identifier2901888624506535
dc.identifier3637285622123132
dc.identifier26303042
dc.identifier0000-0003-2440-8097
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780537
dc.descriptionMost crude venom from Polybiapaulista is composed of short, linear peptides; however, only five of these peptides are structurally and functionally characterized. Therefore, the peptides in this venom were profiled using an HPLC-IT-TOF/MS and MS(n) system. The presence of type -d and -w ions that are generated from the fragmentation of the side chains was used to resolve I/L ambiguity. The distinction between K and Q residues was achieved through esterification of the α- and ε-amino groups in the peptide chains, followed by mass spectrometry analysis. Fourteen major peptides were detected in P. paulista venom and sequenced; all the peptides were synthesized on solid-phase and submitted to a series of bioassays. Five of them had been previously characterized, and nine were novel toxins. The novel peptides correspond to two wasp kinins, two chemotactic components, three mastoparans, and two peptides of unknown function. The seven novel peptides with identified functions appear to act synergistically with the previously known ones, constituting three well-known families of peptide toxins (wasp kinins, chemotactic peptides, and mastoparans) in the venom of social wasps. These multifunctional toxins can cause pain, oedema formation, haemolysis, chemotaxis of PMNLs, and mast cell degranulation in victims who are stung by wasps.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenadação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionUniversidade Estadual Paulista, Departamento de Biologia, Instituto de Biociências de Rio Claro
dc.descriptionUniversidade Estadual Paulista, Centro de Estudos de Insetos Sociais de Rio Claro
dc.descriptionCAPES: 1208/2011
dc.languageeng
dc.publisherElsevier B. V.
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectEsi-it-tof/ms
dc.subjectPeptidomics
dc.subjectPolycationic peptides
dc.subjectRp-hplc
dc.subjectWasp venom
dc.titlePeptidome profiling of venom from the social wasp Polybia paulista
dc.typeArtigo


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