dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorAstolphi, Rafael Dias [UNESP]
dc.creatorCurbete, Mariane Machado [UNESP]
dc.creatorChiba, Fernando Yamamoto [UNESP]
dc.creatorCintra, Luciano Tavares Angelo
dc.creatorErvolino, Edilson [UNESP]
dc.creatorMota, Max Sander de Oliveira da[UNESP]
dc.creatorAntoniali, Cristina [UNESP]
dc.creatorGarbin, Cléa Adas Saliba [UNESP]
dc.creatorSumida, Doris Hissako [UNESP]
dc.date2015-12-07T15:32:05Z
dc.date2015-12-07T15:32:05Z
dc.date2015
dc.date.accessioned2023-09-12T07:30:14Z
dc.date.available2023-09-12T07:30:14Z
dc.identifierhttp://dx.doi.org/10.1016/j.joen.2015.04.002
dc.identifierJournal Of Endodontics, v. 41, n. 8, p. 1305-1310, 2015.
dc.identifier1878-3554
dc.identifierhttp://hdl.handle.net/11449/131154
dc.identifier10.1016/j.joen.2015.04.002
dc.identifier9235743081667362
dc.identifier4408095517346846
dc.identifier8275401688702343
dc.identifier4419158525709686
dc.identifier26027876
dc.identifier0000-0003-4859-0583
dc.identifier0000-0001-5069-8812
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780435
dc.descriptionSerum inflammatory cytokines derived from oral inflammation are associated with decreased insulin signaling (IS) and insulin resistance, which is a major risk factor for type 2 diabetes mellitus. This study aimed to investigate IS in the liver and skeletal muscle (SM) and disorders related to the serum lipid profile and glucose and insulin levels of nondiabetic rats with induced chronic periapical lesions (PLs). Twenty-eight Wistar rats were divided into control and PL groups. PLs were induced by exposing the pulpal tissue to the oral environment. Experiments were conducted in both groups 30 days after pulp exposure. Maxillae were processed for histopathological analysis. IS was evaluated according to insulin receptor substrate (pp185-insulin receptor substrate 1 [IRS-1]/insulin receptor substrate 2 [IRS-2]) tyrosine phosphorylation status, IRS-1 serine phosphorylation status, and IRS-1 and IRS-2 content in the liver and SM by Western blotting. Serum total cholesterol, triglyceride, glucose, and insulin levels were measured enzymatically using a commercial kit. PL rats showed reduced pp185 P-Tyr and increased IRS-1 serine phosphorylation status in the SM but no change in the liver after insulin stimulation. No significant changes in IRS-1 and IRS-2 content, serum total cholesterol, triglyceride, glucose or insulin levels were noted. PLs are associated with decreased insulin signaling in the SM of rats. Because a decrease in insulin signaling is associated with insulin resistance, our results emphasize the importance of preventing local inflammatory diseases such as PLs to prevent alterations in IS in muscle.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionDepartment of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
dc.descriptionDepartment of Endodontics, Araçatuba Dental School, Araçatuba, São Paulo, Brazil.
dc.descriptionDepartment of Child and Social Dentistry, Araçatuba Dental School, UNESP, Araçatuba, São Paulo, Brazil.
dc.descriptionDepartment of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
dc.descriptionDepartment of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
dc.descriptionDepartment of Child and Social Dentistry, Araçatuba Dental School, UNESP, Araçatuba, São Paulo, Brazil.
dc.descriptionDepartment of Basic Sciences, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
dc.descriptionFAPESP: 2012/08722-2
dc.format1305-1310
dc.languageeng
dc.publisherElsevier B. V.
dc.relationJournal Of Endodontics
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectInsulin receptor substrate proteins
dc.subjectLipidemia
dc.subjectPeriapical lesions
dc.subjectSkeletal muscle
dc.titlePeriapical lesions decrease insulin signaling in rat skeletal muscle
dc.typeArtigo


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