Validation of mechanical, electrical and thermal nociceptive stimulation methods in horses

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorTaylor Monroe, Ely, Cambridgeshire, UK.
dc.creatorLuna, S. P. L. [UNESP]
dc.creatorLopes, C. [UNESP]
dc.creatorRosa, A. C. [UNESP]
dc.creatorOliveira, F. A. [UNESP]
dc.creatorCrosignani, N. [UNESP]
dc.creatorTaylor, P. M.
dc.creatorPantoja, J. C. [UNESP]
dc.date2015-12-07T15:31:52Z
dc.date2015-12-07T15:31:52Z
dc.date2015
dc.date.accessioned2023-09-12T07:29:51Z
dc.date.available2023-09-12T07:29:51Z
dc.identifierhttp://dx.doi.org/10.1111/evj.12332
dc.identifierEquine Veterinary Journal, v. 47, n. 5, p. 609-614, 2015.
dc.identifier2042-3306
dc.identifierhttp://hdl.handle.net/11449/131131
dc.identifier10.1111/evj.12332
dc.identifier4473260410099623
dc.identifier25130475
dc.identifier0000-0001-5312-9076
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8780412
dc.descriptionTo validate a model for investigating the effects of analgesic drugs on mechanical, thermal and electrical stimulation testing. To investigate repeatability, sensitivity and specificity of nociceptive tests. Randomised experiment with 2 observers in 2 phases. Mechanical (M), thermal (TL) and electrical (E) stimuli were applied to the dorsal metacarpus (M-left and TL-right) and coronary band of the left thoracic limb (E) and a thoracic thermal stimulus (TT) was applied caudal to the withers in 8 horses (405 ± 43 kg). Stimuli intensities were increased until a clear avoidance response was detected without exceeding 20 N (M), 60°C (TL and TT) and 15 V (E). For each set of tests, 3 real stimuli and one sham stimulus were applied (32 per animal) using a blinded, randomised, crossover design repeated after 6 months. A distribution frequency and, for each stimulus, Chi-square and McNemar tests compared both the proportion of positive responses detected by 2 observers and the 2 study phases. The κ coefficients estimated interobserver agreement in determining endpoints. Sensitivity (384 tests) and specificity (128 tests) were evaluated for each nociceptive stimulus to assess the evaluators' accuracy in detecting real and sham stimuli. Nociceptive thresholds were 3.1 ± 2 N (M), 8.1 ± 3.8 V (E), 51.4 ± 5.5°C (TL) and 55.2 ± 5.3°C (TT). The level of agreement after all tests, M, E, TL and TT, was 90, 100, 84, 98 and 75%, respectively. Sensitivity was 89, 100, 89, 98 and 70% and specificity 92, 97, 88, 91 and 94%, respectively. The high interobserver agreement, sensitivity and specificity suggest that M, E and TL tests are valid for pain studies in horses and are suitable tools for investigating antinociceptive effects of analgesics in horses.
dc.descriptionDepartment of Veterinary Surgery and Anesthesiology, Faculty of Veterinary Medicine and Animal Science, Univ Estadual Paulista, UNESP, Botucatu SP, Brazil.
dc.descriptionDepartment of Anesthesiology, Faculty of Medicine, Univ Estadual Paulista, UNESP, Botucatu SP, Brazil.
dc.descriptionTaylor Monroe, Ely, Cambridgeshire, UK.
dc.descriptionDepartment of Veterinary Hygiene and Public Health, Faculty of Veterinary Medicine and Animal Science, Univ Estadual Paulista, UNESP, Botucatu SP, Brazil.
dc.descriptionDepartment of Veterinary Surgery and Anesthesiology, Faculty of Veterinary Medicine and Animal Science, Univ Estadual Paulista, UNESP, Botucatu SP, Brazil.
dc.descriptionDepartment of Anesthesiology, Faculty of Medicine, Univ Estadual Paulista, UNESP, Botucatu SP, Brazil.
dc.descriptionDepartment of Veterinary Hygiene and Public Health, Faculty of Veterinary Medicine and Animal Science, Univ Estadual Paulista, UNESP, Botucatu SP, Brazil.
dc.format609-614
dc.languageeng
dc.publisherEVJ Ltd.
dc.relationEquine Veterinary Journal
dc.rightsAcesso restrito
dc.sourcePubMed
dc.subjectElectrical stimulus
dc.subjectHorse
dc.subjectMechanical stimulus
dc.subjectThermal stimulus
dc.subjectThreshold
dc.subjectValidation
dc.titleValidation of mechanical, electrical and thermal nociceptive stimulation methods in horses
dc.typeArtigo


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