Diazepam, em dose única, inibe a migração celular, a estimulação macrofágica e a atividade de TNF-α na reação inflamatória aguda induzida por LPS em camundongos

Diazepam, in a single dose, inhibits cellular chemotaxis, macrophage stimulation, and TNF-α activity in LPS-induced acute inflammatory responses in mice

dc.contributorUniversidade Federal de São Carlos (UFSCar)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorMonteiro, Diana Amaral
dc.creatorCarlos, Iracilda Zeppone [UNESP]
dc.creatorPinto, Fábio Gonçalves
dc.date2014-05-27T11:23:41Z
dc.date2014-05-27T11:23:41Z
dc.date2008-10-01
dc.date.accessioned2023-09-12T07:16:16Z
dc.date.available2023-09-12T07:16:16Z
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-93322008000400007&lng=pt&nrm=iso&tlng=pt
dc.identifierRevista Brasileira de Ciências Farmaceuticas. São Paulo: Univ São Paulo, Conjunto Quimicas, v. 44, n. 4, p. 613-620, 2008.
dc.identifier1516-9332
dc.identifierhttp://hdl.handle.net/11449/130436
dc.identifier10.1590/S1516-93322008000400007
dc.identifierS1516-93322008000400007
dc.identifierWOS:000263804500007
dc.identifier2-s2.0-59649119535
dc.identifier2-s2.0-59649119535.pdf
dc.identifier1730146818754269
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8779740
dc.descriptionBenzodiazepines are one of the most frequently prescribed drugs due to their anxiolytic properties. The aim of this study was to evaluate the effects of diazepam on lipopolysaccharide-induced peritoneal acute inflammatory responses. Swiss mice were treated with diazepam in a single dose of 1 or 10 mg/kg- subcutaneously 1 h before an intraperitoneal injection of lipopolysaccharide or sterile saline solution. The mice were killed 16 h after and the cells were washed from the peritoneal cavity to determine the total number of cells and the mononuclear and polimorfonuclear subpopulations, as well as the TNF-alpha activity and percentage of spread macrophages. Our results showed that the diazepam treatment (1 and 10 mg/kg) induced a significant reduction in the LPS-induced macrophage stimulation and TNF-α activity. Diazepam (10 mg/kg) also reduced the inflammatory cellular migration when compared to the control. It can be concluded that the diazepam treatment in a single dose is able to influence the inflammatory cellular influx, macrophage stimulation and TNF-α activity in the acute inflammatory response in mice, having possible implications on the anti-infectious response efficiency.
dc.descriptionDepartamento de Morfologia e Patologia Universidade Federal de São Carlos
dc.descriptionDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista Júlio de Mesquita Filho
dc.descriptionDepartamento de Morfologia e Patologia Centro de Ciências Biológicas e da Saúde Universidade Federal de São Carlos, Rodovia Washington Luiz, km 235, 13565-905 - São Carlos - SP
dc.descriptionDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista Júlio de Mesquita Filho
dc.format613-620
dc.languagepor
dc.publisherUniversidade de São Paulo (USP), Conjunto Quimicas
dc.relationRevista Brasileira de Ciências Farmacêuticas
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectAcute inflammatory response
dc.subjectCellular migration/inhibition
dc.subjectDiazepam
dc.subjectLipopolysaccharide
dc.subjectMacrophages/stimulation
dc.subjectTnf-α
dc.subjectDiazepam
dc.subjectTumor necrosis factor alpha
dc.subjectAnimal cell
dc.subjectAnimal experiment
dc.subjectAntiinflammatory activity
dc.subjectAscites fluid cytology
dc.subjectCell count
dc.subjectChemotaxis
dc.subjectControlled study
dc.subjectDrug dose comparison
dc.subjectDrug effect
dc.subjectDrug mechanism
dc.subjectFemale
dc.subjectInflammation
dc.subjectInflammatory cell
dc.subjectMacrophage
dc.subjectMacrophage migration
dc.subjectMononuclear cell
dc.subjectMouse
dc.subjectNonhuman
dc.subjectPolymorphonuclear cell
dc.subjectSingle drug dose
dc.subjectSwiss webster mouse
dc.titleDiazepam, em dose única, inibe a migração celular, a estimulação macrofágica e a atividade de TNF-α na reação inflamatória aguda induzida por LPS em camundongos
dc.titleDiazepam, in a single dose, inhibits cellular chemotaxis, macrophage stimulation, and TNF-α activity in LPS-induced acute inflammatory responses in mice
dc.typeArtigo


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