Contrasting effects of nitric oxide and corticotropin-releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in mice

dc.contributorUniversidade Federal de São Carlos (UFSCar)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorMiguel, T. T. [UNESP]
dc.creatorGomes, K. S. [UNESP]
dc.creatorNunes-de-Souza, R. L. [UNESP]
dc.date2014-05-20T15:30:46Z
dc.date2014-05-20T15:30:46Z
dc.date2012-04-01
dc.date.accessioned2023-09-12T07:16:15Z
dc.date.available2023-09-12T07:16:15Z
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400003&lng=en&nrm=iso&tlng=en
dc.identifierBrazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 45, n. 4, p. 299-307, 2012.
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/11449/130435
dc.identifier10.1590/S0100-879X2012007500043
dc.identifierS0100-879X2012000400003
dc.identifierWOS:000302556500003
dc.identifierS0100-879X2012000400003.pdf
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8779739
dc.descriptionThe anxiogenic and antinociceptive effects produced by glutamate N-methyl-D-aspartate receptor activation within the dorsal periaqueductal gray (dPAG) matter have been related to nitric oxide (NO) production, since injection of NO synthase (NOS) inhibitors reverses these effects. dPAG corticotropin-releasing factor receptor (CRFr) activation also induces anxiety-like behavior and antinociception, which, in turn, are selectively blocked by local infusion of the CRF type 1 receptor (CRFr1) antagonist, NBI 27914 [5-chloro-4-(N-(cyclopropyl)methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl) aminopyridine]. Here, we determined whether i) the blockade of the dPAG by CRFr1 attenuates the anxiogenic/antinociceptive effects induced by local infusion of the NO donor, NOC-9 [6-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-hexanamine], and ii) the anxiogenic/antinociceptive effects induced by intra-dPAG CRF are prevented by local infusion of N.-propyl-L-arginine (NPLA), a neuronal NOS inhibitor, in mice. Male Swiss mice (12 weeks old, 25-35 g, N = 8-14/group) were stereotaxically implanted with a 7-mm cannula aimed at the dPAG. Intra-dPAG NOC-9 (75 nmol) produced defensive-like behavior (jumping and running) and antinociception (assessed by the formalin test). Both effects were reversed by prior local infusion of NBI 27914 (2 nmol). Conversely, intra-dPAG NPLA (0.4 nmol) did not modify the anxiogenic/antinociceptive effects of CRF (150 pmol). These results suggest that CRFr1 plays an important role in the defensive behavior and antinociception produced by NO within the dPAG. In contrast, the anxiogenic and antinociceptive effects produced by intra-dPAG CRF are not related to NO synthesis in this limbic midbrain structure.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionUniversidade Federal de São Carlos (UFSCar), Programa Interinst Posgrad Ciencias Fisiol, Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, Farmacol Lab, Fac Ciencias Farmaceut, Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, Farmacol Lab, Fac Ciencias Farmaceut, Araraquara, SP, Brazil
dc.descriptionCNPQ: 73102/2009-9
dc.descriptionCAPES: AUX-PE-PNPD-2748/2010
dc.descriptionFAPESP: 2005/05171-1
dc.descriptionFAPESP: 2010/01290-4
dc.descriptionCNPq: 303580/2009-7
dc.format299-307
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relationBrazilian Journal of Medical and Biological Research
dc.relation1.492
dc.rightsAcesso aberto
dc.sourceSciELO
dc.subjectNitric oxide
dc.subjectCorticotropin-releasing factor
dc.subjectPeriaqueductal gray
dc.subjectDefensive behavior
dc.subjectAntinociception
dc.subjectMice
dc.titleContrasting effects of nitric oxide and corticotropin-releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in mice
dc.typeArtigo


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