A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

dc.contributorFaculdade de Medicina de Jundiaí (FMJ)
dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorMachado, Eduardo Gomes
dc.creatorIssa, João Paulo Mardegan
dc.creatorFigueiredo, Fellipe Augusto Tocchini de
dc.creatorSantos, Geovane Ribeiro dos
dc.creatorGaldeano, Ewerton Alexandre
dc.creatorAlves, Mariana Carla
dc.creatorChacon, Erivelto Luis
dc.creatorFerreira Junior, Rui Seabra [UNESP]
dc.creatorBarraviera, Benedito [UNESP]
dc.creatorCunha, Marcelo Rodrigues da
dc.date2015-10-21T21:00:41Z
dc.date2015-10-21T21:00:41Z
dc.date2015-01-01
dc.date.accessioned2023-09-12T06:53:58Z
dc.date.available2023-09-12T06:53:58Z
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0065128115000549
dc.identifierActa Histochemica. Jena: Elsevier Gmbh, Urban &fischer Verlag, v. 117, n. 3, p. 288-296, 2015.
dc.identifier0065-1281
dc.identifierhttp://hdl.handle.net/11449/129398
dc.identifier10.1016/j.acthis.2015.03.006
dc.identifierWOS:000354141300009
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8778738
dc.descriptionTissue engineering has special interest in bone tissue aiming at future medical applications Studies have focused on recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins due to the osteogenic properties of rhBMP-2 and the angiogenic characteristic of fraction 1 protein (P-1) extracted from the rubber tree Hevea brasiliensis. Furthermore, heterologous fibrin sealant (FS) has been shown as a promising alternative in regenerative therapies. The aim of this study was to evaluate these substances for the repair of bone defects in rats. A bone defect measuring 3 mm in diameter was created in the proximal metaphysis of the left tibia of 60 rats and was implanted with rhBMP-2 or P-1 in combination with a new heterologous FS derived from snake venom. The animals were divided into six groups: control (unfilled bone defect), rhBMP-2 (defect filled with 5 mu g rhBMP-2), P-1 (defect filled with 5 mu g P-1), FS (defect filled with 8 mu g FS), FS/rhBMP-2 (defect filled with 8 mu g FS and 5 mu g rhBMP-2), FS/P-1 (defect filled with 8 mu g FS and 5 mu g P-1). The animals were sacrificed 2 and 6 weeks after surgery. The newly formed bone projected from the margins of the original bone and exhibited trabecular morphology and a disorganized arrangement of osteocyte lacunae. Immunohistochemical analysis showed intense expression of osteocalcin in all groups. Histometric analysis revealed a significant difference in all groups after 2 weeks (p < 0.05), except for the rhBMP-2 and FS/rhBMP-2 groups (p > 0.05). A statistically significant difference (p < 0.05) was observed in all groups after 6 weeks in relation to the volume of newly formed bone in the surgical area. In conclusion, the new heterologous fibrin sealant was found to be biocompatible and the combination with rhBMP-2 showed the highest osteogenic and osteoconductive capacity for bone healing. These findings suggest a promising application of this combination in the regeneration surgery.
dc.description"Escola de Medicina de Jundiaí, Departamento de Morfologia e Patologia
dc.descriptionUniversidade de São Paulo, Departamento de Morfologia, Fisiologia e Patologia Básica , Faculdade de Odontologia de Ribeirão Preto"
dc.descriptionUniversidade Estadual Paulista, Centro de Estudos de Venenos e Animais Peçonhentos de Botucatu
dc.format288-296
dc.languageeng
dc.publisherElsevier B.V.
dc.relationActa Histochemica
dc.relation1.652
dc.relation0,661
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectFibrin
dc.subjectSealant
dc.subjectScaffold
dc.subjectBMP-2
dc.subjectNatural latex
dc.subjectProtein P-1
dc.titleA new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects
dc.typeArtigo


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