Effect of mucoadhesive polymers on the in vitro performance of insulin-loaded silica nanoparticles: Interactions with mucin and biomembrane models

dc.contributorUniversity of Tras-os Montes e Alto Douro (UTAD)
dc.contributorCentre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Fernando Pessoa (UFP)
dc.contributorBarcelona University
dc.contributorFaculty of Pharmacy, University of Coimbra (FFUC)
dc.contributorUniversity of Coimbra
dc.creatorAndreani, Tatiana
dc.creatorMiziara, Leonardo [UNESP]
dc.creatorLorenzón, Esteban N. [UNESP]
dc.creatorSouza, Ana Luiza R. de [UNESP]
dc.creatorKiill, Charlene P. [UNESP]
dc.creatorFangueiro, Joana F.
dc.creatorGarcia, Maria L.
dc.creatorGremião, Palmira D. [UNESP]
dc.creatorSilva, Amelia M.
dc.creatorSouto, Eliana B.
dc.date2015-10-21T20:19:39Z
dc.date2015-10-21T20:19:39Z
dc.date2015-06-01
dc.date.accessioned2023-09-12T06:48:10Z
dc.date.available2023-09-12T06:48:10Z
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0939641115001605
dc.identifierEuropean Journal Of Pharmaceutics And Biopharmaceutics. Amsterdam: Elsevier Science Bv, v. 93, p. 118-126, 2015.
dc.identifier0939-6411
dc.identifierhttp://hdl.handle.net/11449/129079
dc.identifier10.1016/j.ejpb.2015.03.027
dc.identifierWOS:000356554000012
dc.identifier9129780536724256
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8778419
dc.descriptionThe present paper focuses on the development and characterization of silica nanoparticles (SiNP) coated with hydrophilic polymers as mucoadhesive carriers for oral administration of insulin. SiNP were prepared by sol-gel technology under mild conditions and coated with different hydrophilic polymers, namely, chitosan, sodium alginate or poly(ethylene glycol) (PEG) with low and high molecular weight (PEG 6000 and PEG 20000) to increase the residence time at intestinal mucosa. The mean size and size distribution, association efficiency, insulin structure and insulin thermal denaturation have been determined. The mean nanoparticle diameter ranged from 289 nm to 625 nm with a PI between 0.251 and 0.580. The insulin association efficiency in SiNP was recorded above 70%. After coating, the association efficiency of insulin increased up to 90%, showing the high affinity of the protein to the hydrophilic polymer chains. Circular dichroism (CD) indicated that no conformation changes of insulin structure occurred after loading the peptide into SiNP. Nano-differential scanning calorimetry (nDSC) showed that SiNP shifted the insulin endothermic peak to higher temperatures. The influence of coating on the interaction of nanoparticles with dipalmitoylphosphatidylcholine (DPPC) biomembrane models was also evaluated by nDSC. The increase of AH values suggested a strong association of non-coated SiNP and those PEGylated nanopartides coated with DPPC polar heads by forming hydrogen bonds and/or by electrostatic interaction. The mucoadhesive properties of nanoparticles were examined by studying the interaction with mucin in aqueous solution. SiNP coated with alginate or chitosan showed high contact with mucin. On the other hand, non-coated SiNP and PEGylated SiNP showed lower interaction with mucin, indicating that these nanopartides can interdiffuse across mucus network. The results of the present work provide valuable data in assessing the in vitro performance of insulin-loaded SiNP coated with mucoadhesive polymers. (C) 2015 Elsevier B.V. All rights reserved.
dc.descriptionFundacao para a Ciencia e Tecnologia (FCT, Portugal)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionEuropean Union Funds (FEDER/COMPETE)
dc.descriptionDepartment of Biology and Environment, University of Tras-os Montes e Alto Douro, UTAD, Quinta de Prados, P-5001-801 Vila Real, Portugal
dc.descriptionCentre for Research and Technology of Agro-Environmental and Biological Sciences, CITAB, UTAD, Quinta de Prados, P-5001-801 Vila Real, Portugal
dc.descriptionResearch Centre for Biomedicine (CEBIMED), Fernando Pessoa University (UFP), Praça 9 de Abril, 349, P-4249-004 Porto, Portugal f Department of Physical Chemistry, Faculty of Pharmacy, Barcelona University, Av. Joan XXIII s/n, 08028 Barcelona, Spain
dc.descriptionDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
dc.descriptionCenter for Neuroscience and Cell Biology & Institute for Biomedical Imaging and Life Sciences (CNC-IBILI), University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.
dc.descriptionDepartment of Biochemistry and Chemical Technology, Institute of Chemistry, UNESP, Araraquara, São Paulo, Brazil
dc.descriptionDepartment of Pharmaceutical Sciences, UNESP-Universidade Estadual Paulista, Rodovia Araraquara-Jau, Km. 01, Araraquara, São Paulo, Brazil.
dc.descriptionFCT, Portugal: SFRH/BD/60640/2009
dc.descriptionFCT, Portugal: SFRH/BD/80335/2011
dc.descriptionFEDER/COMPETE: FCOMP-01-0124-FEDER-022696
dc.descriptionFAPESP: 2012/10174-3
dc.descriptionFAPESP: 140758/2011-9
dc.format118-126
dc.languageeng
dc.publisherElsevier B.V.
dc.relationEuropean Journal Of Pharmaceutics And Biopharmaceutics
dc.relation4.491
dc.relation1,342
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectSilica nanoparticles
dc.subjectMucoadhesion
dc.subjectInsulin
dc.subjectOral delivery
dc.subjectBiomembranes
dc.subjectThermal denaturation
dc.titleEffect of mucoadhesive polymers on the in vitro performance of insulin-loaded silica nanoparticles: Interactions with mucin and biomembrane models
dc.typeArtigo


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