Evaluation of gene expression SAP5, LIP9, and PLB2 of candida albicans biofilms after photodynamic inactivation

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorFreire, Fernanda [UNESP]
dc.creatorBarros, Patricia Pimentel de [UNESP]
dc.creatorAvila, Damara da Silva [UNESP]
dc.creatorBack Brito, Graziella Nuernberg [UNESP]
dc.creatorJunqueira, Juliana Campos [UNESP]
dc.creatorCardoso Jorge, Antonio Olavo [UNESP]
dc.date2015-10-21T13:15:07Z
dc.date2015-10-21T13:15:07Z
dc.date2015-07-01
dc.date.accessioned2023-09-12T06:42:48Z
dc.date.available2023-09-12T06:42:48Z
dc.identifierhttp://link.springer.com/article/10.1007%2Fs10103-015-1747-0
dc.identifierLasers In Medical Science, v. 30, n. 5, p. 1511-1518, 2015.
dc.identifier0268-8921
dc.identifierhttp://hdl.handle.net/11449/128890
dc.identifier10.1007/s10103-015-1747-0
dc.identifierWOS:000356531400013
dc.identifier0322020541055900
dc.identifier0053567153623569
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8778234
dc.descriptionWith the increasing number of strains of Candida ssp. resistant to antifungal agents, the accomplishment of researches that evaluate the effects of new therapeutic methods, like photodynamic inactivation (PDI), becomes important and necessary. Thus, the objective of this study was to verify the effects of the PDI on Candida albicans biofilms, evaluating their effects on the expression of the gene hydrolytic enzymes aspartyl proteinase (SAP5), lipase (LIP9), and phospholipase (PLB2). Clinical strains of C. albicans (n = 9) isolated from patient bearers of the virus HIV and a pattern strain ATCC 18804 were used. The quantification of gene expression was related to the production of hydrolytic enzymes using the quantitative polymerase chain reaction (qPCR) assay. For PDI, we used laser-aluminum-gallium arsenide low power (red visible, 660 nm) as a light source and the methylene blue at 300 mu M as a photosensitizer. We assessed two experimental groups for each strain: (a) PDI: sensitization with methylene blue and laser irradiation and (b) control: without sensitization with methylene blue and light absence. The PDI decreased gene expression in 60 % of samples for gene SAP5 and 50 % of the samples decreased expression of LIP9 and PLB2. When we compared the expression profile for of each gene between the treated and control group, a decrease in all gene expression was observed, however no statistically significant difference (Tukey's test/p = 0.12). It could be concluded that PDI (photosensitization with methylene blue followed by low-level laser irradiation) showed a slight reduction on the expression of hydrolytic enzymes of C. albicans, without statistical significance.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionUniv Estadual Paulista UNESP, Inst Sci &Technol, Dept Biosci &Oral Diag, BR-12245000 Sao Paulo, Brazil
dc.descriptionUniv Estadual Paulista UNESP, Inst Sci &Technol, Dept Biosci &Oral Diag, BR-12245000 Sao Paulo, Brazil
dc.descriptionFAPESP: 2012/09188-0
dc.format1511-1518
dc.languageeng
dc.publisherSpringer
dc.relationLasers In Medical Science
dc.relation1.949
dc.relation0,713
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBiofilm
dc.subjectCandida albicans
dc.subjectGenes of hydrolytic enzymes
dc.subjectPhotodynamic inactivation
dc.subjectqPCR
dc.titleEvaluation of gene expression SAP5, LIP9, and PLB2 of candida albicans biofilms after photodynamic inactivation
dc.typeArtigo


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