dc.contributor | Universidade Federal do Rio Grande do Sul (UFRS) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.creator | Silva, Alessandra Dutra da | |
dc.creator | Lima, Celina Faig [UNESP] | |
dc.creator | Maraschin, Bruna Jalfim | |
dc.creator | Laureano, Natalia Koerich | |
dc.creator | Daroit, Natalia Batista | |
dc.creator | Brochier, Fernanda | |
dc.creator | Sant'Ana Filho, Manoel | |
dc.creator | Visioli, Fernanda | |
dc.creator | Rados, Pantelis Varvaki | |
dc.date | 2015-10-21T13:15:06Z | |
dc.date | 2015-10-21T13:15:06Z | |
dc.date | 2015-04-01 | |
dc.date.accessioned | 2023-09-12T06:42:47Z | |
dc.date.available | 2023-09-12T06:42:47Z | |
dc.identifier | http://www.aqch.com/toc/auto_abstract.php?id=23335 | |
dc.identifier | Analytical And Quantitative Cytopathology And Histopathology. St Louis: Sci Printers &publ Inc, v. 37, n. 2, p. 134-138, 2015. | |
dc.identifier | 0884-6812 | |
dc.identifier | http://hdl.handle.net/11449/128889 | |
dc.identifier | WOS:000356102100007 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8778233 | |
dc.description | Objective: To evaluate different immunocytochemical protocol variations to find the most effective protocol for the analysis of involucrin, epidermal growth factor receptor (EGFR), and E-cadherin antibodies. Exfoliative cytology is a noninvasive method used to monitor and screen for early changes in the oral mucosa of patients exposed to carcinogens such as tobacco and alcohol. It has been postulated that its association with immunocytochemistry may improve the effectiveness of the screening process. Study design: Four graduate students from Porto Alegre in southern Brazil had oral smears collected from the border of the tongue using a cytobrush. The following variables were analyzed: cell membrane permeability, antigen retrieval method (microwave oven or water bath), antibody incubation time (overnight or 1 hour), detection system used (Envision or LSAB), and chromogen incubation time (10 seconds or 5 minutes). Results: Best results were obtained with the following combinations: (1) for involucrin: water bath, 1-hour incubation for primary antibody, Envision, and chromogen incubation for 10 seconds; (2) for EGFR: microwave, overnight incubation, LSAB, and chromogen incubation for 5 minutes; and (3) for E-cadherin: water bath, overnight incubation, Envision, and chromogen incubation for 5 minutes. Conclusion: Our findings suggest that each antibody requires a specific immunocytochemical protocol to guarantee optimal results with oral smears. | |
dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description | Universidade Federal do Rio Grande do Sul, Departamento de Patologia Oral | |
dc.description | Universidade Estadual Paulista, Departamento de Biociências e Diagnóstico Bucal, Insituto de Ciência e Tecnologia de São José dos Campos | |
dc.format | 134-138 | |
dc.language | eng | |
dc.publisher | Sci Printers &publ Inc | |
dc.relation | Analytical And Quantitative Cytopathology And Histopathology | |
dc.relation | 0.398 | |
dc.relation | 0,227 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | E-cadherin antibodies | |
dc.subject | Epidermal growth factor receptor (EGER) | |
dc.subject | Exfoliative cytology | |
dc.subject | Immunocytochemistry | |
dc.subject | Involucrin | |
dc.subject | oral cytopathology | |
dc.subject | Oral exfoliative cytology | |
dc.subject | Oral smears | |
dc.title | Immunocytochemistry associated with oral exfoliative cytology methodological analysis | |
dc.type | Artigo | |