Immunocytochemistry associated with oral exfoliative cytology methodological analysis

dc.contributorUniversidade Federal do Rio Grande do Sul (UFRS)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorSilva, Alessandra Dutra da
dc.creatorLima, Celina Faig [UNESP]
dc.creatorMaraschin, Bruna Jalfim
dc.creatorLaureano, Natalia Koerich
dc.creatorDaroit, Natalia Batista
dc.creatorBrochier, Fernanda
dc.creatorSant'Ana Filho, Manoel
dc.creatorVisioli, Fernanda
dc.creatorRados, Pantelis Varvaki
dc.date2015-10-21T13:15:06Z
dc.date2015-10-21T13:15:06Z
dc.date2015-04-01
dc.date.accessioned2023-09-12T06:42:47Z
dc.date.available2023-09-12T06:42:47Z
dc.identifierhttp://www.aqch.com/toc/auto_abstract.php?id=23335
dc.identifierAnalytical And Quantitative Cytopathology And Histopathology. St Louis: Sci Printers &publ Inc, v. 37, n. 2, p. 134-138, 2015.
dc.identifier0884-6812
dc.identifierhttp://hdl.handle.net/11449/128889
dc.identifierWOS:000356102100007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8778233
dc.descriptionObjective: To evaluate different immunocytochemical protocol variations to find the most effective protocol for the analysis of involucrin, epidermal growth factor receptor (EGFR), and E-cadherin antibodies. Exfoliative cytology is a noninvasive method used to monitor and screen for early changes in the oral mucosa of patients exposed to carcinogens such as tobacco and alcohol. It has been postulated that its association with immunocytochemistry may improve the effectiveness of the screening process. Study design: Four graduate students from Porto Alegre in southern Brazil had oral smears collected from the border of the tongue using a cytobrush. The following variables were analyzed: cell membrane permeability, antigen retrieval method (microwave oven or water bath), antibody incubation time (overnight or 1 hour), detection system used (Envision or LSAB), and chromogen incubation time (10 seconds or 5 minutes). Results: Best results were obtained with the following combinations: (1) for involucrin: water bath, 1-hour incubation for primary antibody, Envision, and chromogen incubation for 10 seconds; (2) for EGFR: microwave, overnight incubation, LSAB, and chromogen incubation for 5 minutes; and (3) for E-cadherin: water bath, overnight incubation, Envision, and chromogen incubation for 5 minutes. Conclusion: Our findings suggest that each antibody requires a specific immunocytochemical protocol to guarantee optimal results with oral smears.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionUniversidade Federal do Rio Grande do Sul, Departamento de Patologia Oral
dc.descriptionUniversidade Estadual Paulista, Departamento de Biociências e Diagnóstico Bucal, Insituto de Ciência e Tecnologia de São José dos Campos
dc.format134-138
dc.languageeng
dc.publisherSci Printers &publ Inc
dc.relationAnalytical And Quantitative Cytopathology And Histopathology
dc.relation0.398
dc.relation0,227
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectE-cadherin antibodies
dc.subjectEpidermal growth factor receptor (EGER)
dc.subjectExfoliative cytology
dc.subjectImmunocytochemistry
dc.subjectInvolucrin
dc.subjectoral cytopathology
dc.subjectOral exfoliative cytology
dc.subjectOral smears
dc.titleImmunocytochemistry associated with oral exfoliative cytology methodological analysis
dc.typeArtigo


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