Molecular diversity of fungal and bacterial communities in the marine sponge Dragmacidon reticulatum

dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorPassarini, Michel R. Z.
dc.creatorMiqueletto, Paula B.
dc.creatorOliveira, Valeria M. de
dc.creatorSette, Lara D. [UNESP]
dc.date2015-10-21T13:12:47Z
dc.date2015-10-21T13:12:47Z
dc.date2015-02-01
dc.date.accessioned2023-09-12T06:39:45Z
dc.date.available2023-09-12T06:39:45Z
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/jobm.201400466/abstract
dc.identifierJournal Of Basic Microbiology. Hoboken: Wiley-blackwell, v. 55, n. 2, p. 207-220, 2015.
dc.identifier0233-111X
dc.identifierhttp://hdl.handle.net/11449/128728
dc.identifier10.1002/jobm.201400466
dc.identifierWOS:000348564200008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8778074
dc.descriptionThe present work aimed to investigate the diversity of bacteria and filamentous fungi of southern Atlantic Ocean marine sponge Dragmacidon reticulatum using cultivation-independent approaches. Fungal ITS rDNA and 18S gene analyses (DGGE and direct sequencing approaches) showed the presence of representatives of three order (Polyporales, Malasseziales, and Agaricales) from the phylum Basidiomycota and seven orders belonging to the phylum Ascomycota (Arthoniales, Capnodiales, Dothideales, Eurotiales, Hypocreales, Pleosporales, and Saccharomycetales). On the other hand, bacterial 16S rDNA gene analyses by direct sequencing approach revealed the presence of representatives of seven bacterial phyla (Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Lentisphaerae, Chloroflexi, and Planctomycetes). Results from statistical analyses (rarefaction curves) suggested that the sampled clones covered the fungal diversity in the sponge samples studied, while for the bacterial community additional sampling would be necessary for saturation. This is the first report related to the molecular analyses of fungal and bacterial communities by cultivation-independent approaches in the marine sponges D. reticulatum. Additionally, the present work broadening the knowledge of microbial diversity associated to marine sponges and reports innovative data on the presence of some fungal genera in marine samples.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionUniv Estadual Campinas, CPQBA, Ctr Pluridisciplinar Pesquisas Quim Biol &Agr, Div Recursos Microbianos,UNICAMP, Paulinia, SP, Brazil
dc.descriptionUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Bioquim &Microbiol, BR-13506900 Rio Claro, SP, Brazil
dc.descriptionUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Bioquim &Microbiol, BR-13506900 Rio Claro, SP, Brazil
dc.descriptionFAPESP: 2008/06720-7
dc.descriptionFAPESP: 2010/50190-2
dc.format207-220
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationJournal Of Basic Microbiology
dc.relation1.580
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectMarine sponge
dc.subjectMicrobial diversity
dc.subjectCultivation-independent approach
dc.subjectDGGE
dc.subjectDirect sequencing
dc.titleMolecular diversity of fungal and bacterial communities in the marine sponge Dragmacidon reticulatum
dc.typeArtigo


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