The noradrenergic projection from the locus coeruleus to the cochlear root neurons in rats

dc.contributorUniversity of Salamanca
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorHormigo, Sebastián
dc.creatorGómez-Nieto, Ricardo
dc.creatorCastellano, Orlando
dc.creatorHerrero-Turrion, Manuel Javier
dc.creatorLopez, Dolores Estilita
dc.creatorHorta-Júnior, José de Anchieta de Castro e [UNESP]
dc.date2015-10-21T13:10:51Z
dc.date2015-10-21T13:10:51Z
dc.date2015-05-01
dc.date.accessioned2023-09-12T06:36:27Z
dc.date.available2023-09-12T06:36:27Z
dc.identifierhttp://link.springer.com/article/10.1007%2Fs00429-014-0739-3
dc.identifierBrain Structure &function. Heidelberg: Springer Heidelberg, v. 220, n. 3, p. 1477-1496, 2015.
dc.identifier1863-2653
dc.identifierhttp://hdl.handle.net/11449/128547
dc.identifier10.1007/s00429-014-0739-3
dc.identifierWOS:000353515200017
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8777897
dc.descriptionThe cochlear root neurons (CRNs) are key components of the primary acoustic startle circuit; mediating auditory alert and escape behaviors in rats. They receive a great variety of inputs which serve to elicit and modulate the acoustic startle reflex (ASR). Recently, our group has suggested that CRNs receive inputs from the locus coeruleus (LC), a noradrenergic nucleus which participates in attention and alertness. Here, we map the efferent projection patterns of LC neurons and confirm the existence of the LC-CRN projection using both anterograde and retrograde tract tracers. Our results show that each LC projects to the CRNs of both sides with a clear ipsilateral predominance. The LC axons terminate as small endings distributed preferentially on the cell body and primary dendrites of CRNs. Using light and confocal microscopy, we show a strong immunoreactivity for tyrosine hydroxylase and dopamine beta-hydroxylase in these terminals, indicating noradrenaline release. We further studied the noradrenergic system using gene expression analysis (RT-qPCR) and immunohistochemistry to detect specific noradrenergic receptor subunits in the cochlear nerve root. Our results indicate that CRNs contain a noradrenergic receptor profile sufficient to modulate the ASR, and also show important gender-specific differences in their gene expression. 3D reconstruction analysis confirms the presence of sexual dimorphism in the density and distribution of LC neurons. Our study describes a coerulean noradrenergic projection to the CRNs that might contribute to neural processes underlying sensory gating of the ASR, and also provides an explanation for the gender differences observed in the behavioral paradigm.
dc.descriptionSpanish Ministry of Science and Innovation (MICINN)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionUniversity of Salamanca, Neuroscience Institute of Castilla y León
dc.descriptionUniversity of Salamanca, Institute of Biomedical Research of Salamanca
dc.descriptionUniversity of Salamanca, Department of Cell Biology and Pathology
dc.descriptionUniversidade Estadual Paulista, Departamento de Anatomia, Instituto de Biociências de Botucatu
dc.descriptionSpanish Ministry of Science and Innovation (MICINN): BFU2010-17754
dc.descriptionFAPESP: 2008/02771-6
dc.format1477-1496
dc.languageeng
dc.publisherSpringer
dc.relationBrain Structure &function
dc.relation4.231
dc.relation2,034
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAcoustic startle reflex
dc.subjectGender differences
dc.subjectNeuronal tracers
dc.subjectNoradrenergic receptors
dc.subjectSensory gating
dc.subject3D reconstruction
dc.titleThe noradrenergic projection from the locus coeruleus to the cochlear root neurons in rats
dc.typeArtigo


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