Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributorUniversidade Estadual do Norte do Paraná (UENP)
dc.contributorUniversidade de São Paulo (USP)
dc.creatorMartinez, Paula Felippe [UNESP]
dc.creatorBonomo, Camila [UNESP]
dc.creatorGuizoni, Daniele Mendes [UNESP]
dc.creatorOliveira Junior, Silvio Assis [UNESP]
dc.creatorDamatto, Ricardo Luiz [UNESP]
dc.creatorCezar, Marcelo Diarcadia Mariano [UNESP]
dc.creatorLima, Aline Regina Ruiz [UNESP]
dc.creatorPagan, Luana Urbano [UNESP]
dc.creatorSeiva, Fabio Rodrigues
dc.creatorFernandes, Denise Castro
dc.creatorLaurindo, Francisco Rafael Martins
dc.creatorNovelli, Ethel Lourenzi Barbosa [UNESP]
dc.creatorMatsubara, Luiz Shiguero [UNESP]
dc.creatorZornoff, Leonardo Antonio Mamede [UNESP]
dc.creatorOkoshi, Katashi [UNESP]
dc.creatorOkoshi, Marina Politi [UNESP]
dc.date2015-10-21T13:08:47Z
dc.date2015-10-21T13:08:47Z
dc.date2015-01-01
dc.date.accessioned2023-09-12T06:32:00Z
dc.date.available2023-09-12T06:32:00Z
dc.identifierhttp://www.karger.com/Article/FullText/369683
dc.identifierCellular Physiology And Biochemistry. Basel: Karger, v. 35, n. 1, p. 148-159, 2015.
dc.identifier1015-8987
dc.identifierhttp://hdl.handle.net/11449/128297
dc.identifier10.1159/000369683
dc.identifierWOS:000348048000014
dc.identifierWOS000348048000014.pdf
dc.identifier6309835137998766
dc.identifier1590971576309420
dc.identifier4463138671998432
dc.identifier5016839015394547
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8777649
dc.descriptionBackground: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI, rats were assigned to Sham, MI-C (without treatment), and MI-NAC (treated with N-acetylcysteine) groups. Two months later, echocardiogram showed left ventricular dysfunction in MI-C; NAC attenuated diastolic dysfunction. In soleus muscle, glutathione peroxidase and superoxide dismutase activity was decreased in MI-C and unchanged by NAC. 3-nitrotyrosine was similar in MI-C and Sham, and lower in MI-NAC than MI-C. Total reactive oxygen species (ROS) production was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products. The 2-hydroxyethidium (EOH)/DHE ratio did not differ between Sham and MI-C and was higher in MI-NAC. The ethidium/DHE ratio was higher in MI-C than Sham and unchanged by NAC. NADPH oxidase activity was similar in Sham and MI-C and lower in MI-NAC. Gene expression of p47(phox) was lower in MI-C than Sham. NAC decreased NOX4 and p22(phox) expression. Conclusions: We corroborate the case that oxidative stress is increased in skeletal muscle of heart failure rats and show for the first time that oxidative stress is not related to increased NADPH oxidase activity.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.descriptionUniversidade de São Paulo, Instituto do Coração do Hospital das Clínicas, Faculdade de Medicina
dc.descriptionUniversidade Estadual Paulista, Departamento de Clínica Médica, Faculdade de Medicina de Botucatu
dc.descriptionFAPESP: 2010/50461-6
dc.descriptionFAPESP: 2007/59500-1
dc.descriptionCNPq: 306857/2012-0
dc.descriptionCNPq: 306.845/2012-1
dc.format148-159
dc.languageeng
dc.publisherKarger
dc.relationCellular Physiology And Biochemistry
dc.relation5.500
dc.relation1,561
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectHeart failure
dc.subjectSkeletal muscle
dc.subjectNADPH oxidase
dc.subjectMyocardial infarction
dc.subjectN-acetylcysteine
dc.subjectReactive oxygen species
dc.titleInfluence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
dc.typeArtigo


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