Analysis of Steroids using Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry-Mass Spectrometry (SPME-GC-MS-MS)

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade de São Paulo (USP)
dc.creatorChopra, Shilpi
dc.creatorLima Gomes, Paulo Clairmont Feitosa de [UNESP]
dc.creatorDhandapani, Ramkumar
dc.creatorSnow, Nicholas H.
dc.date2015-05-15T13:30:23Z
dc.date2015-05-15T13:30:23Z
dc.date2014
dc.date.accessioned2023-09-12T05:02:56Z
dc.date.available2023-09-12T05:02:56Z
dc.identifierhttp://www.scientiachromatographica.com/doi/10.4322/sc.2014.024
dc.identifierScientia Chromatographica, v. 6, n. 2, p. 105-116, 2014.
dc.identifier1984-4433
dc.identifierhttp://hdl.handle.net/11449/123538
dc.identifier10.4322/sc.2014.024
dc.identifier49857977635051
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8772974
dc.descriptionDirect immersion SPME-GC-MS-MS was used for the analysis of steroids in water at part-per-trillion(ppt) and lower concentrations. The method was validated and extended to real sample analysis. The method were linear from 0.01 to 5 ng/ml with precision less than 10% relative standard deviation for a steroid mixture at 1 ng/ml. Limit of quantitation and limit of detection was found to be 200- 1200 pg/L and 30-200 pg/L respectively and recoveries ranged from 88-103 %. To understand the extraction efficiency of the fiber, a depletion study was performed. The fiber/ sample partition coefficients for the steroids were determined to be 1.0 x 104 to 1.5 x 104 . The extraction was performed without derivatization or the use of an internal standard. SPMEGC-MS-MS effectively demonstrated ultra-trace level detection of steroids in water.
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Química de Araraquara, Araraquara, Rua Professor Francisco Degni, Departamento de Química Analítica, Jardim Quitandinha, CEP 14800060, SP, Brasil
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Química de Araraquara, Araraquara, Rua Professor Francisco Degni, Departamento de Química Analítica, Jardim Quitandinha, CEP 14800060, SP, Brasil
dc.format105-116
dc.languagepor
dc.relationScientia Chromatographica
dc.rightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectSteroids using Solid Phase Microextraction
dc.subjectSPME
dc.subjectGas Chromatography-Mass Spectrometry-Mass Spectrometry
dc.subjectGC-MS-MS
dc.subjectSPMEGC-MS-MS
dc.subjectwater analysis
dc.titleAnalysis of Steroids using Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry-Mass Spectrometry (SPME-GC-MS-MS)
dc.typeArtigo


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