Study of pulmonary experimental paracoccidioidomycosis by analysis of bronchoalveolar lavage cells: Resistant vs. susceptible mice

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorVilani-Moreno, F.
dc.creatorFecchio, Denise
dc.creatorde Mattos, MCI
dc.creatorMoscardi-Bacchi, M.
dc.creatorFaveri, Julio de
dc.creatorFranco, M.
dc.date2014-05-20T15:22:00Z
dc.date2016-10-25T17:55:35Z
dc.date2014-05-20T15:22:00Z
dc.date2016-10-25T17:55:35Z
dc.date1998-01-01
dc.date.accessioned2017-04-05T23:34:31Z
dc.date.available2017-04-05T23:34:31Z
dc.identifierMycopathologia. Dordrecht: Kluwer Academic Publ, v. 141, n. 2, p. 79-91, 1998.
dc.identifier0301-486X
dc.identifierhttp://hdl.handle.net/11449/33066
dc.identifierhttp://acervodigital.unesp.br/handle/11449/33066
dc.identifier10.1023/A:1006987205848
dc.identifierWOS:000075825700005
dc.identifierhttp://dx.doi.org/10.1023/A:1006987205848
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/877270
dc.descriptionAdult Swiss (susceptible) and BALB/c (non-susceptible) mice were inoculated by the intravenous route with 1 x 10(6) yeast cells of Paracoccidioides brasiliensis, strain 18. Immunologic parameters, histopathology and features of the bronchoalveolar lavage (BAL) were evaluated at week 2, 4, 8 and 16 post-infection. The pulmonary infection was progressive in Swiss mice and regressive in BALB/c mice. The numbers of total cells, lymphocytes and polymorphonuclear neutrophils increased in BAL, as well as the percentages of giant cells, and CD4 and CD8 positive cells. The ultrastructural study of BAL cells revealed a predominance of macrophages and a frequency of 13.2% of type II pneumocytes. As the infection progressed, the number of fungal cells and spreading macrophages, as well as the stimulated release of H2O2 by macrophages, increased. The animals exhibited an exacerbation of the humoral immune response and a depression of cellular immunity during the infection. There was a good correlation between the intensity and the pattern of the pulmonary histopathology and the cellular findings in the BAL. The present model reproduces some anatomoclinical patterns of the human disease and shows that BAL may be a useful tool in monitoring the pulmonary infection caused by P. brasiliensis.
dc.languageeng
dc.publisherKluwer Academic Publ
dc.relationMycopathologia
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbronchoalveolar lavage
dc.subjectparacoccidioidomycosis
dc.titleStudy of pulmonary experimental paracoccidioidomycosis by analysis of bronchoalveolar lavage cells: Resistant vs. susceptible mice
dc.typeOtro


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