Potential utility of melatonin as an antioxidant therapy in the management of sickle cell anemia

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.creatorSilva, Danilo Grünig Humberto da [UNESP]
dc.creatorRicci Júnior, Octávio
dc.creatorAlmeida, Eduardo Alves de [UNESP]
dc.creatorBonini-Domingos, Claudia Regina [UNESP]
dc.date2015-04-27T11:56:07Z
dc.date2015-04-27T11:56:07Z
dc.date2014
dc.date.accessioned2023-09-12T04:48:15Z
dc.date.available2023-09-12T04:48:15Z
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/jpi.12204/abstract
dc.identifierJournal of Pineal Research, v. 58, n. 2, p. 178-188, 2014.
dc.identifier0742-3098
dc.identifierhttp://hdl.handle.net/11449/122879
dc.identifier10.1111/jpi.12204
dc.identifier6713400866382255
dc.identifier3279428066176719
dc.identifier0000-0002-4603-9467
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8772322
dc.descriptionThis study aimed to assess antioxidant effects of melatonintreatment compared to N-acetylcysteine (NAC) and to their combination in asickle cell suspension. Sickle erythrocytes were suspended in phosphate-buffered saline, pH 7.4, composing external control group. They were alsosuspended and incubated at 37°C either in the absence (experimental controlgroup) or in the presence of NAC, melatonin and their combination atconcentrations of 100 pM, 100 nM and 100 lM for 1 hr (treatment groups).The melatonin influences were evaluated by spectrophotometric [hemolysisdegree, catalase (CAT), glutathione S-transferase (GST), glutathioneperoxidase (GPx), glutathione reductase (GR), glucose-6-phosphatedehydrogenase (G6PDH), and superoxide dismutase (SOD) activities] andchromatographic methods [glutathione (GSH) and malondialdehyde (MDA)levels]. Incubation period was able to cause a rise about 64% on hemolysisdegree as well as practically doubled the lipid peroxidation levels (P < 0.01).However, almost all antioxidants tested treatments neutralized this incubationeffect observed in MDA levels. Among the antioxidant biomarkers evaluated,we observed a modulating effect of combined treatment on GPx and SODactivities (P < 0.01), which showed ~25% decrease in their activities. Inaddition, we found an antioxidant dose-dependent effect for melatonin onlipid peroxidation (r = 0.29; P = 0.03) and for combined antioxidanttreatments also on MDA levels (r = 0.37; P = 0.01) and on SOD activity(r = 0.54; P < 0.01). Hence, these findings contribute with important insightthat melatonin individually or in combination with NAC may be useful forsickle cell anemia management.
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, Rua Cristóvão Colombo, 2265, Jardim Nazareth, CEP 15054-000, SP, Brasil
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto
dc.format178-188
dc.languageeng
dc.relationJournal of Pineal Research
dc.relation11.613
dc.relation3,945
dc.rightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectalternative therapy
dc.subjectantioxidant capacity
dc.subjecthemoglobin S
dc.subjectmelatonin
dc.subjectoxidative stress
dc.titlePotential utility of melatonin as an antioxidant therapy in the management of sickle cell anemia
dc.typeArtigo


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