Discovery of a new antiviral protein isolated Lonomia obliqua analysed by bioinformatics and real-time approaches

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorCarmo, Ana Carolina Viegas
dc.creatorYamasaki, Lilian Hiromi Tomanari
dc.creatorFigueiredo, Cristina Adelaide
dc.creatorGiovanni, Dalton Nogueira da Silva
dc.creatorOliveira, Maria Isabel de
dc.creatorSantos, Fabiana Cristina Pereira dos
dc.creatorCurti, Suely Pires
dc.creatorRahal, Paula [UNESP]
dc.creatorMendonça, Ronaldo Zucatelli
dc.date2015-04-27T11:55:59Z
dc.date2015-04-27T11:55:59Z
dc.date2014
dc.date.accessioned2023-09-12T04:45:30Z
dc.date.available2023-09-12T04:45:30Z
dc.identifierhttp://link.springer.com/article/10.1007%2Fs10616-014-9740-1
dc.identifierCytotechnology, p. 01-11, 2014.
dc.identifier0920-9069
dc.identifierhttp://hdl.handle.net/11449/122733
dc.identifier10.1007/s10616-014-9740-1
dc.identifier7991082362671212
dc.identifier5993626657930796
dc.identifier5565216743065412
dc.identifier0000-0001-5693-6148
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8772182
dc.descriptionThis study presents a new recombinant protein that acts as a powerful antiviral (rAVLO—recombinant Antiviral protein of Lonomia obliqua). It was able to reduce the replication by 106 fold for herpes virus and by 104 fold for rubella virus. RT-PCR of viral RNA rAVLO treated infected cells also showed similar rate of inhibition in replication. The analysis of this protein by bioinformatics suggests that this protein is globular, secreted with a signal peptide and has the ability to bind to MHC class I. It was found that there are several protein binding sites with various HLA and a prevalence of α-helices in the N-terminal region (overall classified as a α/β protein type). BLAST similarity sequence search for corresponding cDNA did not reveal a similar sequence in Genbank, suggesting that it is from a novel protein family. In this study we have observed that this recombinant protein and hemolymph has a potent antiviral action. This protein was produced in a baculovirus/Sf-9 system. Therefore, these analyses suggest that this novel polypeptide is a candidate as a broad spectrum antiviral.
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, São José do Rio Preto, Rua Cristóvão Colombo - 2265, Jardim Nazareth, CEP 15054000, SP, Brasil
dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, São José do Rio Preto, Rua Cristóvão Colombo - 2265, Jardim Nazareth, CEP 15054000, SP, Brasil
dc.format01-11
dc.languageeng
dc.relationCytotechnology
dc.relation1.461
dc.relation0,519
dc.rightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectLonomia
dc.titleDiscovery of a new antiviral protein isolated Lonomia obliqua analysed by bioinformatics and real-time approaches
dc.typeArtigo


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