Immunocytochemical localization of heparin in secretory granules of rat peritoneal mast cells using a monoclonal anti-heparin antibody (ST-1)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOliani, S. M.
dc.creatorFreymuller, E.
dc.creatorTakahashi, H. K.
dc.creatorStraus, A. H.
dc.date2014-05-20T15:21:15Z
dc.date2016-10-25T17:54:37Z
dc.date2014-05-20T15:21:15Z
dc.date2016-10-25T17:54:37Z
dc.date1997-02-01
dc.date.accessioned2017-04-05T23:30:19Z
dc.date.available2017-04-05T23:30:19Z
dc.identifierJournal of Histochemistry & Cytochemistry. Seattle: Histochemical Soc Inc., v. 45, n. 2, p. 231-235, 1997.
dc.identifier0022-1554
dc.identifierhttp://hdl.handle.net/11449/32410
dc.identifierhttp://acervodigital.unesp.br/handle/11449/32410
dc.identifier10.1177/002215549704500208
dc.identifierWOS:A1997WM75700008
dc.identifierhttp://dx.doi.org/10.1177/002215549704500208
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/876761
dc.descriptionWe performed immunogold labeling with an ST-1 monoclonal antibody (IgM), specific for intact heparin, to define the subcellular localization of heparin in mast cells. Rat peritoneal mast cells were fixed by a modified Karnovsky method and embedded in Araldite. Ultrathin sections were first treated with sodium periodate and then sequentially incubated with MAb ST-1, rabbit anti-mouse IgM, and protein A-gold. By transmission electron microscopy, gold particles were localized inside cytoplasmic granules of peritoneal mast cells. In contrast, with the same procedure, no labeling was observed in mast cells from rat intestinal mucosa. Control sections of rat peritoneal or intestinal mucosa mast Mast cells cells treated with an irrelevant MAb (IgM) did not show any labeling. Treatment with nitrous Heparin acid abolished the reactivity of MAb ST-1 with peritoneal mast cells. These results Granules show that different mast cells can be identified regarding their heparin content by immunochemical procedures using MAb ST-1.
dc.languageeng
dc.publisherHistochemical Soc Inc
dc.relationJournal of Histochemistry & Cytochemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMast cells
dc.subjectheparin
dc.subjectgranules
dc.subjectimmunolabeling
dc.subjectmonoclonal antibody
dc.subjectelectron microscopy
dc.titleImmunocytochemical localization of heparin in secretory granules of rat peritoneal mast cells using a monoclonal anti-heparin antibody (ST-1)
dc.typeOtro


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