Assay of Serum Glutamic-Oxaloacetic Transaminase Using Malate Dehydrogenase Preparation Obtained from Streptomyces aureofaciens

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorYamanaka, H.
dc.creatorLaluce, C.
dc.creatorDeoliveiraneto, G.
dc.date2014-05-20T15:21:13Z
dc.date2016-10-25T17:54:36Z
dc.date2014-05-20T15:21:13Z
dc.date2016-10-25T17:54:36Z
dc.date1995-01-01
dc.date.accessioned2017-04-05T23:30:14Z
dc.date.available2017-04-05T23:30:14Z
dc.identifierAnalytical Letters. New York: Marcel Dekker Inc., v. 28, n. 13, p. 2305-2316, 1995.
dc.identifier0003-2719
dc.identifierhttp://hdl.handle.net/11449/32395
dc.identifierhttp://acervodigital.unesp.br/handle/11449/32395
dc.identifier10.1080/00032719508000374
dc.identifierWOS:A1995RV75900005
dc.identifierhttp://dx.doi.org/10.1080/00032719508000374
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/876752
dc.descriptionA modified spectrophotometric method for serum glutamic-oxaloacetic transaminase (SGOT) assay was developed. A crude cell-free extract from Streptomyces aureofaciens which showed a high level of malate dehydrogenase (MDH) activity (E.C. 1.1.1.37) was used as the enzymatic indicator. The lyophilized microbial preparation was used without previous purification and was quite stable under refrigeration for one year. Serum sample assays using both the method utilizing the crude cell extract and an enzymatic commercial kit showed good correlation.
dc.languageeng
dc.publisherMarcel Dekker Inc
dc.relationAnalytical Letters
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTRANSAMINASE
dc.subjectSGOT
dc.subjectMALATE DEHYDROGENASE
dc.subjectSTREPTOMYCES
dc.titleAssay of Serum Glutamic-Oxaloacetic Transaminase Using Malate Dehydrogenase Preparation Obtained from Streptomyces aureofaciens
dc.typeOtro


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