Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Federal de São Carlos (UFSCar)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorRosario Natl Univ
dc.creatorLeite, Aline Lima
dc.creatorVaz Madureira Lobo, Janete Gualiume
dc.creatorBarbosa da Silva Pereira, Heloisa Aparecida
dc.creatorFernandes, Mileni Silva
dc.creatorMartini, Tatiani
dc.creatorZucki, Fernanda
dc.creatorSumida, Doris Hissako [UNESP]
dc.creatorRigalli, Alfredo
dc.creatorRabelo Buzalaf, Marilia Afonso
dc.date2015-03-18T15:56:03Z
dc.date2015-03-18T15:56:03Z
dc.date2014-09-02
dc.date.accessioned2023-09-12T03:11:33Z
dc.date.available2023-09-12T03:11:33Z
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0106646
dc.identifierPlos One. San Francisco: Public Library Science, v. 9, n. 9, 10 p., 2014.
dc.identifier1932-6203
dc.identifierhttp://hdl.handle.net/11449/117404
dc.identifier10.1371/journal.pone.0106646
dc.identifierWOS:000341231500120
dc.identifierWOS000341231500120.pdf
dc.identifier8275401688702343
dc.identifier2740250822457796
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8766888
dc.descriptionAdministration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin-induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionUniv Sao Paulo, Bauru Dent Sch, Dept Biol Sci, Bauru, SP, Brazil
dc.descriptionUniv Fed Sao Carlos, Ctr Biol Sci & Hlth, Dept Genet & Evolut, BR-13560 Sao Carlos, SP, Brazil
dc.descriptionUniv Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP, Brazil
dc.descriptionRosario Natl Univ, Sch Med, Natl Sci & Tech Res Council CONICET, Bone Biol & Mineral Metab Lab, Rosario, Santa Fe, Argentina
dc.descriptionUniv Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP, Brazil
dc.descriptionFAPESP: 10/17736-1
dc.format10
dc.languageeng
dc.publisherPublic Library Science
dc.relationPlos One
dc.relation2.766
dc.relation1,164
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.titleProteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride
dc.typeArtigo


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