Green tea extract and its major constituent, epigallocatechin-3-gallate, induce epithelial beta-defensin secretion and prevent beta-defensin degradation by Porphyromonas gingivalis

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniv Laval
dc.contributorSichuan Univ
dc.creatorLombardo Bedran, T. B. [UNESP]
dc.creatorFeghali, K.
dc.creatorZhao, L.
dc.creatorPalomari Spolidorio, D. M. [UNESP]
dc.creatorGrenier, D.
dc.date2015-03-18T15:55:20Z
dc.date2015-03-18T15:55:20Z
dc.date2014-10-01
dc.date.accessioned2023-09-12T03:06:45Z
dc.date.available2023-09-12T03:06:45Z
dc.identifierhttp://dx.doi.org/10.1111/jre.12142
dc.identifierJournal Of Periodontal Research. Hoboken: Wiley-blackwell, v. 49, n. 5, p. 615-623, 2014.
dc.identifier0022-3484
dc.identifierhttp://hdl.handle.net/11449/117146
dc.identifier10.1111/jre.12142
dc.identifierWOS:000341884200009
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8766631
dc.descriptionBackground and Objective: Antimicrobial peptides, such as beta-defensins, secreted by gingival epithelial cells, are thought to play a major role in preventing periodontal diseases. In the present study, we investigated the ability of green tea polyphenols to induce human beta-defensin (hBD) secretion in gingival epithelial cells and to protect hBDs from proteolytic degradation by Porphyromonas gingivalis.Material and Methods: Gingival epithelial cells were treated with various amounts (25-200 mu g/mL) of green tea extract or epigallocatechin-3-gallate (EGCG). The secretion of hBD1 and hBD2 was measured using ELISAs, and gene expression was quantified by real-time PCR. The treatments were also carried out in the presence of specific kinase inhibitors to identify the signaling pathways involved in hBD secretion. The ability of green tea extract and EGCG to prevent hBD degradation by proteases of P. gingivalis present in a bacterial culture supernatant was evaluated by ELISA.Results: The secretion of hBD1 and hBD2 was up-regulated, in a dose-dependent manner, following the stimulation of gingival epithelial cells with a green tea extract or EGCG. Expression of the hBD gene in gingival epithelial cells treated with green tea polyphenols was also increased. EGCG-induced secretion of hBD1 and hBD2 appeared to involve extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase. Lastly, green tea extract and EGCG prevented the degradation of recombinant hBD1 and hBD2 by a culture supernatant of P. gingivalis.Conclusion: Green tea extract and EGCG, through their ability to induce hBD secretion by epithelial cells and to protect hBDs from proteolytic degradation by P. gingivalis, have the potential to strengthen the epithelial antimicrobial barrier. Future clinical studies will indicate whether these polyphenols represent a valuable therapeutic agent for treating/preventing periodontal diseases.
dc.descriptionCanadian Institutes of Health Research
dc.descriptionState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, Araraquara, SP, Brazil
dc.descriptionUniv Laval, Fac Dent, Oral Ecol Res Grp, Quebec City, PQ G1V 0A6, Canada
dc.descriptionSichuan Univ, West China Hosp Stomatol, Dept Periodont, Chengdu 610064, Sichuan, Peoples R China
dc.descriptionState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, Araraquara, SP, Brazil
dc.format615-623
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationJournal Of Periodontal Research
dc.relation2.878
dc.relation0,927
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbeta-defensin
dc.subjectepigallocatechin gallate
dc.subjectepithelial cells
dc.subjectgreen tea
dc.subjectperiodontal disease
dc.subjectPorphyromonas gingivalis
dc.titleGreen tea extract and its major constituent, epigallocatechin-3-gallate, induce epithelial beta-defensin secretion and prevent beta-defensin degradation by Porphyromonas gingivalis
dc.typeArtigo


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