Antibiofilm activity of irrigating solutions associated with cetrimide. Confocal laser scanning microscopy

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade de São Paulo (USP)
dc.creatorGuerreiro-Tanomaru, J. M. [UNESP]
dc.creatorNascimento, C. A. [UNESP]
dc.creatorFaria-Junior, N. B. [UNESP]
dc.creatorGraeff, M. S. Z.
dc.creatorWatanabe, E.
dc.creatorTanomaru-Filho, M. [UNESP]
dc.date2015-03-18T15:55:13Z
dc.date2015-03-18T15:55:13Z
dc.date2014-11-01
dc.date.accessioned2023-09-12T03:06:12Z
dc.date.available2023-09-12T03:06:12Z
dc.identifierhttp://dx.doi.org/10.1111/iej.12248
dc.identifierInternational Endodontic Journal. Hoboken: Wiley-blackwell, v. 47, n. 11, p. 1058-1063, 2014.
dc.identifier0143-2885
dc.identifierhttp://hdl.handle.net/11449/117113
dc.identifier10.1111/iej.12248
dc.identifierWOS:000343760600006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8766598
dc.descriptionAimTo evaluate the antibiofilm activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) solutions associated with cetrimide (CTR), and QMiX using confocal laser scanning microscopy.MethodologyEnterococcus faecalis (ATCC- 29212) biofilms were induced on bovine dentine blocks for 14days. The dentine blocks containing biofilm were immersed for 1min in the following solutions: 2.5% NaOCl; 2.5% NaOCl+0.2% CTR; 2% CHX; 2% CHX+0.2% CTR; 0.2% CTR; QMiX. After contact with the solutions, the dentine blocks were stained with Live/Dead((R)) BacLight for analysis of the remaining biofilm using confocal laser scanning microscope. Images were evaluated using the BioImage_L software to determine the total biovolume (m(3)), the green biovolume (live cells) (m(3)) and the percentage of substrate coverage (%). The data were subjected to nonparametric statistical test using Kruskal-Wallis and Dunn's tests at 5% significance level.ResultsAfter exposure to irrigants, the total biovolume observed for CHX, CHX+CTR, CTR, QMiX was similar to distilled water (P>0.05). NaOCl and NaOCl+CTR had the lowest total and green biovolume. The CTR and QMiX had intermediate green biovolume, with greater antibacterial activity than CHX and CHX+CTR (P<0.05). The NaOCl and NaOCl+CTR solutions were associated with microorganism removal and substrate cleaning ability.ConclusionsNaOCl and NaOCl+CTR solutions were effective on microorganism viability and were able to eliminate biofilm. The addition of cetrimide did not influence antibacterial activity.
dc.descriptionUNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil
dc.descriptionUniv Sao Paulo, Bauru Dent Sch, Integrated Res Ctr, Bauru, SP, Brazil
dc.descriptionUniv Sao Paulo, Ribeirao Preto Dent Sch, Dept Restorat Dent, Ribeirao Preto, SP, Brazil
dc.descriptionUNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil
dc.format1058-1063
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationInternational Endodontic Journal
dc.relation3.015
dc.relation1,791
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbiofilm
dc.subjectchlorhexidine
dc.subjectconfocal laser scanning microscopy
dc.subjectEnterococcus faecalis
dc.subjectsodium hypochlorite
dc.titleAntibiofilm activity of irrigating solutions associated with cetrimide. Confocal laser scanning microscopy
dc.typeArtigo


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