Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorMato Grosso Fed Inst
dc.contributorUniversidade Estadual de Maringá (UEM)
dc.creatorCosta, Daryne L. M. G. [UNESP]
dc.creatorRinaldo, Daniel [UNESP]
dc.creatorVaranda, Eliana A. [UNESP]
dc.creatorSousa, Juliana F. de [UNESP]
dc.creatorNasser, Ana L. M. [UNESP]
dc.creatorSilva, Ana C. Z. [UNESP]
dc.creatorBaldoqui, Debora C.
dc.creatorVilegas, Wagner [UNESP]
dc.creatorSantos, Lourdes Campaner dos [UNESP]
dc.date2015-03-18T15:54:42Z
dc.date2015-03-18T15:54:42Z
dc.date2014-10-01
dc.date.accessioned2023-09-12T03:03:20Z
dc.date.available2023-09-12T03:03:20Z
dc.identifierhttp://dx.doi.org/10.1089/jmf.2013.0116
dc.identifierJournal Of Medicinal Food. New Rochelle: Mary Ann Liebert, Inc, v. 17, n. 10, p. 1103-1112, 2014.
dc.identifier1096-620X
dc.identifierhttp://hdl.handle.net/11449/116991
dc.identifier10.1089/jmf.2013.0116
dc.identifierWOS:000342563300008
dc.identifier3538253640602977
dc.identifier0000-0003-3032-2556
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8766476
dc.descriptionIt is well known that phytotherapy has grown in popularity in recent years. Because a drug cannot be administered without ensuring its effectiveness and safety, the standardization and regulation of phytotherapeutic drugs are required by the global market and governmental authorities. This article describes a simple and reliable high-performance liquid chromatography-diode array detection analysis method for the simultaneous detection of myricetin-3-O-beta-D-galactopyranoside, myricetin-3-O-alpha-L-arabinopyranoside, and myricetin-3-O-alpha-L-rhaminopyranoside present in the hydroethanolic extract (ethanol/H2O, 7:3, v/v) of Pouteria torta. The mutagenic activity of the extract was evaluated on Salmonella typhimurium and by an in vivo micronucleus test on the peripheral blood cells of Swiss mice. The linearity, sensitivity, selectivity, repeatability, accuracy, and precision of the assay were evaluated. The analytical curves were linear and exhibited good repeatability (with a deviation of less than 5%) and demonstrated good recovery (within the 83-107% range). The results demonstrate that the hydroethanolic extract exhibited a mutagenic activity in both assays, suggesting caution in the use of this plant in folk medicine.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionSao Paulo State Univ, Inst Chem, Dept Organ Chem, UNESP, BR-14800900 Sao Paulo, Brazil
dc.descriptionMato Grosso Fed Inst, IFMT, Cuiaba, Mato Grosso, Brazil
dc.descriptionSao Paulo State Univ, Dept Chem, Fac Sci, UNESP, BR-14800900 Sao Paulo, Brazil
dc.descriptionSao Paulo State Univ, Fac Pharmaceut Sci, Dept Biol Sci, UNESP, BR-14800900 Sao Paulo, Brazil
dc.descriptionUniv Estadual Maringa, Dept Chem, UEM, Maringa, Parana, Brazil
dc.descriptionSao Paulo State Univ, UNESP, BR-14800900 Sao Paulo, Brazil
dc.descriptionSao Paulo State Univ, Inst Chem, Dept Organ Chem, UNESP, BR-14800900 Sao Paulo, Brazil
dc.descriptionSao Paulo State Univ, Dept Chem, Fac Sci, UNESP, BR-14800900 Sao Paulo, Brazil
dc.descriptionSao Paulo State Univ, Fac Pharmaceut Sci, Dept Biol Sci, UNESP, BR-14800900 Sao Paulo, Brazil
dc.descriptionSao Paulo State Univ, UNESP, BR-14800900 Sao Paulo, Brazil
dc.format1103-1112
dc.languageeng
dc.publisherMary Ann Liebert, Inc
dc.relationJournal Of Medicinal Food
dc.relation1.954
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmutagenicity
dc.subjectliquid chromatography
dc.subjectPouteria torta
dc.subjectflavonoids
dc.subjectquantification
dc.titleFlavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity
dc.typeArtigo


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