Phototherapy up-regulates dentin matrix proteins expression and synthesis by stem cells from human-exfoliated deciduous teeth

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorTurrioni, Ana Paula S. [UNESP]
dc.creatorBasso, Fernanda G. [UNESP]
dc.creatorMontoro, Liege A. [UNESP]
dc.creatorAlmeida, Leopoldina de Fatima D. de [UNESP]
dc.creatorSouza Costa, Carlos A. de [UNESP]
dc.creatorHebling, Josimeri [UNESP]
dc.date2015-03-18T15:53:33Z
dc.date2015-03-18T15:53:33Z
dc.date2014-10-01
dc.date.accessioned2023-09-09T11:07:18Z
dc.date.available2023-09-09T11:07:18Z
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2014.07.014
dc.identifierJournal Of Dentistry. Oxford: Elsevier Sci Ltd, v. 42, n. 10, p. 1292-1299, 2014.
dc.identifier0300-5712
dc.identifierhttp://hdl.handle.net/11449/116592
dc.identifier10.1016/j.jdent.2014.07.014
dc.identifierWOS:000342361400010
dc.identifier4517484241515548
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8766077
dc.descriptionObjectives: The aim of this study was to evaluate the effects of infrared LED (850 nm) irradiation on dentin matrix proteins expression and synthesis by cultured stem cells from human exfoliated deciduous teeth (SHED).Methods: Near-exfoliation primary teeth were extracted (n = 3), and SHED cultures were characterized by immunofluorescence using STRO-1, CD44, CD146, Nanog and OCT3/4 antibodies, before experimental protocol. The SHEDs were seeded (3 x 10(4) cells/cm(2)) with DMEM containing 10% FBS. After 24-h incubation, the culture medium was replaced by osteogenic differentiation medium, and the cells were irradiated with LED light at energy densities (EDs) of 0 (control), 2, or 4 J/cm(2) (n = 8). The irradiated SHEDs were then evaluated for alkaline phosphatase (ALP) activity, total protein (TP) production, and collagen synthesis (SIRCOL (TM) Assay), as well as ALP, collagen type I (Col I), dentin sialophosphoprotein (DSPP), and dentin matrix acidic phosphoprotein (DMP-1) gene expression (qPCR). Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (alpha = 0.05).Results: Increased ALP activity and collagen synthesis, as well as gene expression of DSPP and ALP, were observed for both EDs compared with non-irradiated cells. The ED of 4 J/cm(2) also increased gene expression of COL I and DMP-1.Conclusions: In conclusion, infrared LED irradiation was capable of biostimulating SHEDs by increasing the expression and synthesis of proteins related with mineralized tissue formation, with overall better results for the energy dose of 4 J/cm(2).Clinical significance: Phototherapy is an additional approach for the clinical application of LED in Restorative Dentistry. Infrared LED irradiation of the cavity's floor could biostimulate subjacent pulp cells, improving local tissue healing. (C) 2014 Elsevier Ltd. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionUniv Estadual Paulista, UNESP, Sch Dent, Dept Pediat Dent & Orthodont, BR-14801903 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, UNESP, Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, UNESP, Sch Dent, Dept Restorat Dent, BR-14801903 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, UNESP, Sch Dent, Dept Pediat Dent & Orthodont, BR-14801903 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, UNESP, Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, UNESP, Sch Dent, Dept Restorat Dent, BR-14801903 Araraquara, SP, Brazil
dc.descriptionFAPESP: 11/13895-0
dc.descriptionFAPESP: 13/17758-3
dc.descriptionCNPq: 301291/2010-1
dc.format1292-1299
dc.languageeng
dc.publisherElsevier B.V.
dc.relationJournal Of Dentistry
dc.relation3.770
dc.relation1,919
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDentin
dc.subjectOdontoblasts
dc.subjectPhototherapy
dc.subjectProtein expression
dc.subjectPulp biology
dc.subjectStem cells
dc.titlePhototherapy up-regulates dentin matrix proteins expression and synthesis by stem cells from human-exfoliated deciduous teeth
dc.typeArtigo


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