dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade de São Paulo (USP)
dc.creatorKalatzis Sousa, Natalia Guimaraes [UNESP]
dc.creatorBarros Cardoso, Cristina Ribeiro de
dc.creatorSilva, Joeio Satana da
dc.creatorKuga, Milton Carlos [UNESP]
dc.creatorTanomaru-Filho, Mario [UNESP]
dc.creatorFaria, Gisele [UNESP]
dc.date2015-03-18T15:53:19Z
dc.date2015-03-18T15:53:19Z
dc.date2014-09-01
dc.date.accessioned2023-09-09T11:04:41Z
dc.date.available2023-09-09T11:04:41Z
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2014.05.021
dc.identifierArchives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 59, n. 9, p. 944-953, 2014.
dc.identifier0003-9969
dc.identifierhttp://hdl.handle.net/11449/116436
dc.identifier10.1016/j.archoralbio.2014.05.021
dc.identifierWOS:000341481600010
dc.identifier1627568419811788
dc.identifier1411074363039082
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8765921
dc.descriptionObjective: To evaluate the expression of matrix metalloproteinase inducer (EMMPRIN) and its correlation with the expression of matrix metalloproteinases (MMPs)-1, -2 and -9 during the development of periapical lesion in mice.Methods: Periapical lesions were induced in the lower first molars of mice and after 7, 14, 21 and 42 days the mandibles were removed. The periapical lesions were measured by micro-computed tomography. The expression of EMMPRIN, MMPs-1, -2, and -9 genes were determined by real-time RT-PCR. The location and expression of EMMPRIN and MMPs were evaluated by immunohistochemistry.Results: At 14 days, the periapical lesion area was higher than at 7 days. At 21 and 42 days no statistically significant bone loss was observed in comparison to 14 days. The control group showed discrete and occasional EMMPRIM, MMP-1, -2 and -9 immunostaining in the periodontal ligament fibroblasts. At 7, 14, 21 and 42 days intense immunoexpression was observed for EMMPRIN, MMPs-1, -2 and -9 in the region adjacent to the apical foramen. The EMMPRIN immunoexpression was higher at 7, 14, 21 and 42 days compared with the control. There was a positive correlation between gene expression of EMMPRIN and MMPs in the active phase of periapical lesion development.Conclusion: There is a high expression of EMMPRIM mainly by the inflammatory infiltrate in the region adjacent to the apical foramen during periapical lesion development. Furthermore, the positive correlation with MMP-1, -2, and -9 during the first days after periapical lesion induction indicates that EMMPRIM may be involved in the active phase of periapical lesions development. (C) 2014 Elsevier Ltd. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionUNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, BR-14801903 Araraquara, SP, Brazil
dc.descriptionUniv Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal Toxicol & Food Sci, BR-14040904 Ribeirao Preto, SP, Brazil
dc.descriptionUniv Sao Paulo, Dept Biochem & Immunol, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP, Brazil
dc.descriptionUNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, BR-14801903 Araraquara, SP, Brazil
dc.descriptionFAPESP: 08/58064-6
dc.descriptionFAPESP: 11/19511-0
dc.format944-953
dc.languageeng
dc.publisherElsevier B.V.
dc.relationArchives Of Oral Biology
dc.relation2.050
dc.relation0,752
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEndodontics
dc.subjectPeriapical lesion
dc.subjectMatrix metalloproteinases
dc.subjectEMMPRIN
dc.titleAssociation of matrix metalloproteinase inducer (EMMPRIN) with the expression of matrix metalloproteinases-1, -2 and -9 during periapical lesion development
dc.typeArtigo


Este ítem pertenece a la siguiente institución