dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade de São Paulo (USP) | |
dc.creator | Kalatzis Sousa, Natalia Guimaraes [UNESP] | |
dc.creator | Barros Cardoso, Cristina Ribeiro de | |
dc.creator | Silva, Joeio Satana da | |
dc.creator | Kuga, Milton Carlos [UNESP] | |
dc.creator | Tanomaru-Filho, Mario [UNESP] | |
dc.creator | Faria, Gisele [UNESP] | |
dc.date | 2015-03-18T15:53:19Z | |
dc.date | 2015-03-18T15:53:19Z | |
dc.date | 2014-09-01 | |
dc.date.accessioned | 2023-09-09T11:04:41Z | |
dc.date.available | 2023-09-09T11:04:41Z | |
dc.identifier | http://dx.doi.org/10.1016/j.archoralbio.2014.05.021 | |
dc.identifier | Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 59, n. 9, p. 944-953, 2014. | |
dc.identifier | 0003-9969 | |
dc.identifier | http://hdl.handle.net/11449/116436 | |
dc.identifier | 10.1016/j.archoralbio.2014.05.021 | |
dc.identifier | WOS:000341481600010 | |
dc.identifier | 1627568419811788 | |
dc.identifier | 1411074363039082 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8765921 | |
dc.description | Objective: To evaluate the expression of matrix metalloproteinase inducer (EMMPRIN) and its correlation with the expression of matrix metalloproteinases (MMPs)-1, -2 and -9 during the development of periapical lesion in mice.Methods: Periapical lesions were induced in the lower first molars of mice and after 7, 14, 21 and 42 days the mandibles were removed. The periapical lesions were measured by micro-computed tomography. The expression of EMMPRIN, MMPs-1, -2, and -9 genes were determined by real-time RT-PCR. The location and expression of EMMPRIN and MMPs were evaluated by immunohistochemistry.Results: At 14 days, the periapical lesion area was higher than at 7 days. At 21 and 42 days no statistically significant bone loss was observed in comparison to 14 days. The control group showed discrete and occasional EMMPRIM, MMP-1, -2 and -9 immunostaining in the periodontal ligament fibroblasts. At 7, 14, 21 and 42 days intense immunoexpression was observed for EMMPRIN, MMPs-1, -2 and -9 in the region adjacent to the apical foramen. The EMMPRIN immunoexpression was higher at 7, 14, 21 and 42 days compared with the control. There was a positive correlation between gene expression of EMMPRIN and MMPs in the active phase of periapical lesion development.Conclusion: There is a high expression of EMMPRIM mainly by the inflammatory infiltrate in the region adjacent to the apical foramen during periapical lesion development. Furthermore, the positive correlation with MMP-1, -2, and -9 during the first days after periapical lesion induction indicates that EMMPRIM may be involved in the active phase of periapical lesions development. (C) 2014 Elsevier Ltd. All rights reserved. | |
dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description | UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, BR-14801903 Araraquara, SP, Brazil | |
dc.description | Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal Toxicol & Food Sci, BR-14040904 Ribeirao Preto, SP, Brazil | |
dc.description | Univ Sao Paulo, Dept Biochem & Immunol, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP, Brazil | |
dc.description | UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Restorat Dent, BR-14801903 Araraquara, SP, Brazil | |
dc.description | FAPESP: 08/58064-6 | |
dc.description | FAPESP: 11/19511-0 | |
dc.format | 944-953 | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Archives Of Oral Biology | |
dc.relation | 2.050 | |
dc.relation | 0,752 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Endodontics | |
dc.subject | Periapical lesion | |
dc.subject | Matrix metalloproteinases | |
dc.subject | EMMPRIN | |
dc.title | Association of matrix metalloproteinase inducer (EMMPRIN) with the expression of matrix metalloproteinases-1, -2 and -9 during periapical lesion development | |
dc.type | Artigo | |