Histopathological study of ovaries of Rhipicephalus sanguineus (Acari: Ixodidae) exposed to different thymol concentrations

dc.contributorUniv Fed Juiz de Fora
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorMatos, Renata da Silva
dc.creatorDaemon, Erik
dc.creatorCamargo-Mathias, Maria Izabel [UNESP]
dc.creatorScopinho Furquim, Karim Christina [UNESP]
dc.creatorSampieri, Bruno Rodrigues [UNESP]
dc.creatorRemedio, Rafael Neodini [UNESP]
dc.creatorAraujo, Laryssa Xavier
dc.creatorLopes Novato, Tatiane Pinheiro
dc.date2015-03-18T15:52:59Z
dc.date2015-03-18T15:52:59Z
dc.date2014-12-01
dc.date.accessioned2023-09-09T11:01:57Z
dc.date.available2023-09-09T11:01:57Z
dc.identifierhttp://dx.doi.org/10.1007/s00436-014-4145-9
dc.identifierParasitology Research. New York: Springer, v. 113, n. 12, p. 4555-4565, 2014.
dc.identifier0932-0113
dc.identifierhttp://hdl.handle.net/11449/116266
dc.identifier10.1007/s00436-014-4145-9
dc.identifierWOS:000344866500029
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8765753
dc.descriptionThymol is a monoterpene with proven acaricide action for several tick species. In addition to killing these ectoparasites, thymol can also reduce oviposition and egg hatch rate. However, the effects of thymol on the morphophysiology of tick ovaries are still unknown. Thus, the aim of this study was to evaluate the morphophysiological changes caused by this active principle in ovaries of Rhipicephalus sanguineus after a 6-day feeding period, through the application of morphohistochemical techniques. After the feeding period, a total of 50 females were divided into five groups and immersed in the following solutions: (I) distilled water (control), (II) 30 % ethanol (control), (III) 1.25 mg/mL thymol, (IV) 2.5 mg/mL thymol, and (V) 5.0 mg/mL thymol. The experimental groups were kept in a climatic chamber (27 +/- 1 A degrees C; RH 80 A +/- 10 %) for 5 days. After this period, morphological (hematoxylin/eosin) and histochemical (von Kossa) techniques were applied after remotion of the ovaries. The morphological results revealed large vacuoles in germ cells at different developmental stages and invaginations that represent deformations in the chorionic membrane. From the results obtained in this study, it was concluded that thymol interfered with the development of oocytes, which showed degeneration signs. The treatment containing 5.0 mg/mL thymol affected more accentuately the morphological development. Moreover, thymol also altered the calcium content of yolk granules, which generally showed an intense staining for this element.
dc.descriptionUniv Fed Juiz de Fora, Programa Posgrad Comportamento & Biol Anim, BR-36036330 Juiz De Fora, MG, Brazil
dc.descriptionUniv Estadual Paulista, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.descriptionUniv Estadual Paulista, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.format4555-4565
dc.languageeng
dc.publisherSpringer
dc.relationParasitology Research
dc.relation2.558
dc.relation0,991
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectHistology
dc.subjectHistochemistry
dc.subjectBrown dog tick
dc.subjectMonoterpene
dc.titleHistopathological study of ovaries of Rhipicephalus sanguineus (Acari: Ixodidae) exposed to different thymol concentrations
dc.typeArtigo


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