Asymmetric chromosome segregation in Xanthomonas citri ssp. citri

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorFundo Defesa Citricultura
dc.creatorUcci, Amanda P. [UNESP]
dc.creatorMartins, Paula M. M. [UNESP]
dc.creatorLau, Ivy F. [UNESP]
dc.creatorBacci, Maurício [UNESP]
dc.creatorBelasque, Jose
dc.creatorFerreira, Henrique [UNESP]
dc.date2015-03-18T15:52:35Z
dc.date2015-03-18T15:52:35Z
dc.date2014-02-01
dc.date.accessioned2023-09-09T11:00:08Z
dc.date.available2023-09-09T11:00:08Z
dc.identifierhttp://dx.doi.org/10.1002/mbo3.145
dc.identifierMicrobiologyopen. Hoboken: Wiley-blackwell, v. 3, n. 1, p. 29-41, 2014.
dc.identifier2045-8827
dc.identifierhttp://hdl.handle.net/11449/116206
dc.identifier10.1002/mbo3.145
dc.identifierWOS:000342352300003
dc.identifierWOS000342352300003.pdf
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8765695
dc.descriptionThis study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB-GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, Brazil
dc.descriptionFundo Defesa Citricultura, BR-14807040 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, Brazil
dc.descriptionFAPESP: 10/02041-8
dc.descriptionFAPESP: 04/09173-6
dc.descriptionFAPESP: 10/05099-7
dc.descriptionCNPq: 142293/2009-1
dc.format29-41
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationMicrobiologyopen
dc.relation2.682
dc.relation1,158
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCell division arrest
dc.subjectchromosome segregation
dc.subjectcitrus canker
dc.titleAsymmetric chromosome segregation in Xanthomonas citri ssp. citri
dc.typeArtigo


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