PURIFICATION AND PROPERTIES OF SHIKIMATE DEHYDROGENASE FROM CUCUMBER (CUCUMIS-SATIVUS L)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLourenco, E. J.
dc.creatorSilva, GML
dc.creatorNeves, Valdir Augusto
dc.date2014-05-20T15:20:58Z
dc.date2016-10-25T17:54:11Z
dc.date2014-05-20T15:20:58Z
dc.date2016-10-25T17:54:11Z
dc.date1991-03-01
dc.date.accessioned2017-04-05T23:28:31Z
dc.date.available2017-04-05T23:28:31Z
dc.identifierJournal of Agricultural and Food Chemistry. Washington: Amer Chemical Soc, v. 39, n. 3, p. 458-462, 1991.
dc.identifier0021-8561
dc.identifierhttp://hdl.handle.net/11449/32155
dc.identifierhttp://acervodigital.unesp.br/handle/11449/32155
dc.identifier10.1021/jf00003a006
dc.identifierWOS:A1991FC49000006
dc.identifierhttp://dx.doi.org/10.1021/jf00003a006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/876546
dc.descriptionShikimate dehydrogenase (SDH, EC 1.1.1.25) extracted from cucumber pulp (Cucumis sativus L.) was purified 7-fold by precipitation with ammonium sulfate and elution from columns of Sephadex G-25, DEAE-cellulose, and hydroxyapatite. Two activity bands were detected on polyacrylamide gel electrophoresis at the last purification step. pH optimum was 8.7, and molecular weight of 45 000 was estimated on a Sephadex G-100 column. SDH was inhibited competitively by protocatechuic acid with a K(i) value of 2 x 10-4 M. K(m) values of 6 x 10-5 and 1 x 10-5 M were determined for shikimic acid and NADP+, respectively. The enzyme was completely inhibited by HgCl2 and p-(chloromercuri)benzoate (PCMB). NaCl and KCl showed partial protection against inhibition by PCMB. Heat inactivation between 50 and 55-degrees-C was biphasic, and the enzyme was completely inactivated after 10 min at 60-degrees-C. Incubation of SDH with either NADP+ or shikimic acid protected the enzyme against heat inactivation.
dc.languageeng
dc.publisherAmer Chemical Soc
dc.relationJournal of Agricultural and Food Chemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titlePURIFICATION AND PROPERTIES OF SHIKIMATE DEHYDROGENASE FROM CUCUMBER (CUCUMIS-SATIVUS L)
dc.typeOtro


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