Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorPinheiro, Luiza
dc.creatorBrito, Carla Ivo
dc.creatorPereira, Valéria Cataneli
dc.creatorOliveira, Adilson De
dc.creatorCamargo, Carlos Henrique
dc.creatorCunha, Maria De Lourdes Ribeiro De Souza Da
dc.date2015-02-02T12:39:36Z
dc.date2015-02-02T12:39:36Z
dc.date2014-11-01
dc.date.accessioned2023-09-09T10:32:55Z
dc.date.available2023-09-09T10:32:55Z
dc.identifierhttp://dx.doi.org/10.1590/0074-0276140120
dc.identifierMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 109, n. 7, p. 871-878, 2014.
dc.identifier0074-0276
dc.identifierhttp://hdl.handle.net/11449/114515
dc.identifier10.1590/0074-0276140120
dc.identifierS0074-02762014000700871
dc.identifierS0074-02762014000700871.pdf
dc.identifier0115647772315973
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8764067
dc.descriptionThis study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of Staphylococcus epidermidis isolated from blood cultures. The isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (SCCmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (MIC) was measured for isolates and subpopulations growing on vancomycin screen agar. The mecA gene was detected in 81.3% of the S. epidermidis isolated and 48.2% carried SCCmec type III. The complete icaADBC operon was observed in 38.3% of the isolates; of these, 58.5% produced a biofilm. Furthermore, 47.7% of the isolates grew on vancomycin screen agar, with an increase in the MIC in 75.9% of the isolates. Determination of the MIC of subpopulations revealed that 64.7% had an MIC ≥ 4 μg mL-1, including 15.7% with an MIC of 8 μg mL-1 and 2% with an MIC of 16 μg mL-1. The presence of the icaADBC operon, biofilm production and reduced susceptibility to vancomycin were associated with methicillin resistance. This study reveals a high level of methicillin resistance, biofilm formation and reduced susceptibility to vancomycin in subpopulations of S. epidermidis. These findings may explain the selection of multidrug-resistant isolates in hospital settings and the consequent failure of antimicrobial treatment.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionUniversidade Estadual Paulista Instituto de Biociências de Botucatu Departamento de Microbiologia e Imunologia
dc.descriptionUniversidade Estadual Paulista Instituto de Biociências de Botucatu Departamento de Microbiologia e Imunologia
dc.format871-878
dc.languageeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relationMemórias do Instituto Oswaldo Cruz
dc.relation2.833
dc.relation1,172
dc.rightsAcesso aberto
dc.sourceSciELO
dc.subjectStaphylococcus epidermidis
dc.subjecticaADBC
dc.subjectmecA
dc.subjectSCCmec
dc.subjectvancomycin
dc.subjectMIC
dc.titleReduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures
dc.typeArtigo


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