Analysis of ibuprofen enantiomers in rat plasma by liquid chromatography with tandem mass spectrometry

dc.contributorUniversidade de São Paulo (USP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorCavalcanti Cardoso, Juciane Lauren
dc.creatorLanchote, Vera Lucia
dc.creatorMarques Pereira, Maria Paula
dc.creatorMoraes, Natália Valadares de [UNESP]
dc.creatorLepera, José Salvador [UNESP]
dc.date2014-12-03T13:11:44Z
dc.date2014-12-03T13:11:44Z
dc.date2014-04-01
dc.date.accessioned2023-09-09T10:16:21Z
dc.date.available2023-09-09T10:16:21Z
dc.identifierhttp://dx.doi.org/10.1002/jssc.201301184
dc.identifierJournal Of Separation Science. Weinheim: Wiley-v C H Verlag Gmbh, v. 37, n. 8, p. 944-949, 2014.
dc.identifier1615-9306
dc.identifierhttp://hdl.handle.net/11449/113482
dc.identifier10.1002/jssc.201301184
dc.identifierWOS:000334059900007
dc.identifier6710074203174471
dc.identifier8087835756545728
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8763079
dc.descriptionA sensitive and selective method for the analysis of ibuprofen enantiomers by LC-MS/MS was developed and validated for the purpose of application in pharmacokinetic studies in small experimental animals. Aliquots of 200 L plasma were submitted to liquid-liquid extraction with hexane/diisopropylether (50:50 v/v) in acid pH. Separation was accomplished in a Chirex (R) 3005 (250 x 4.6 mm, 5 m) column at 25 degrees C with a mobile phase that consisted of 0.01 M ammonium acetate in methanol at a flow rate of 1.1 mL/min. The mass spectrometer consisted of an ESI interface operating at negative ionization mode and multiple reaction monitoring. The transitions 205 > 161 and 240 > 197 were monitored for ibuprofen enantiomers and fenoprofen (internal standard), respectively. Method validation included the evaluation of the matrix effect, stability, linearity, lower LOQ, within-run and between-run precision, and accuracy. The lower LOQ was 25 ng/mL for each ibuprofen enantiomer, and the calibration curves showed good linearity in the range 0.025-50 g/mL. The method was successfully applied in the investigation of pharmacokinetic disposition of ibuprofen enantiomers in rats treated orally with 25 mg/kg of the racemate. Enantioselective kinetic disposition was observed with accumulation of (+)-(S)-ibuprofen in rats following single oral administration.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-05508 Sao Paulo, Brazil
dc.descriptionUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Principios Ativos Nat & Toxicol, BR-14801902 Araraquara, SP, Brazil
dc.descriptionUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Principios Ativos Nat & Toxicol, BR-14801902 Araraquara, SP, Brazil
dc.descriptionFAPESP: 09/17532-0
dc.format944-949
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationJournal of Separation Science
dc.relation2.415
dc.relation0,795
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEnantiomers
dc.subjectIbuprofen
dc.subjectPharmacokinetics
dc.subjectPlasma
dc.titleAnalysis of ibuprofen enantiomers in rat plasma by liquid chromatography with tandem mass spectrometry
dc.typeArtigo


Este ítem pertenece a la siguiente institución