Selective photoinactivation of Candida albicans in the non-vertebrate host infection model Galleria mellonella

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorMassachusetts Gen Hosp
dc.contributorFac Pindamonhangaba
dc.contributorNucl & Energy Res Inst
dc.contributorSouthern Med Univ
dc.contributorSecond Mil Med Univ
dc.contributorUniv New Mexico
dc.contributorHarvard Univ
dc.contributorMIT
dc.contributorBrown University
dc.creatorChibebe Junior, Jose [UNESP]
dc.creatorSabino, Caetano P.
dc.creatorTan, Xiaojiang
dc.creatorJunqueira, Juliana C. [UNESP]
dc.creatorWang, Yan
dc.creatorFuchs, Beth B.
dc.creatorJorge, Antonio O. C. [UNESP]
dc.creatorTegos, George P.
dc.creatorHamblin, Michael R.
dc.creatorMylonakis, Eleftherios
dc.date2014-12-03T13:11:10Z
dc.date2014-12-03T13:11:10Z
dc.date2013-10-01
dc.date.accessioned2023-09-09T10:07:20Z
dc.date.available2023-09-09T10:07:20Z
dc.identifierhttp://dx.doi.org/10.1186/1471-2180-13-217
dc.identifierBmc Microbiology. London: Biomed Central Ltd, v. 13, 9 p., 2013.
dc.identifier1471-2180
dc.identifierhttp://hdl.handle.net/11449/112947
dc.identifier10.1186/1471-2180-13-217
dc.identifierWOS:000325693400001
dc.identifierWOS000325693400001.pdf
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8762562
dc.descriptionBackground: Candida spp. are recognized as a primary agent of severe fungal infection in immunocompromised patients, and are the fourth most common cause of bloodstream infections. Our study explores treatment with photodynamic therapy (PDT) as an innovative antimicrobial technology that employs a nontoxic dye, termed a photosensitizer (PS), followed by irradiation with harmless visible light. After photoactivation, the PS produces either singlet oxygen or other reactive oxygen species (ROS) that primarily react with the pathogen cell wall, promoting permeabilization of the membrane and cell death. The emergence of antifungal-resistant Candida strains has motivated the study of antimicrobial PDT (aPDT) as an alternative treatment of these infections. We employed the invertebrate wax moth Galleria mellonella as an in vivo model to study the effects of aPDT against C. albicans infection. The effects of aPDT combined with conventional antifungal drugs were also evaluated in G. mellonella.Results: We verified that methylene blue-mediated aPDT prolonged the survival of C. albicans infected G. mellonella larvae. The fungal burden of G. mellonella hemolymph was reduced after aPDT in infected larvae. A fluconazole-resistant C. albicans strain was used to test the combination of aPDT and fluconazole. Administration of fluconazole either before or after exposing the larvae to aPDT significantly prolonged the survival of the larvae compared to either treatment alone.Conclusions: G. mellonella is a useful in vivo model to evaluate aPDT as a treatment regimen for Candida infections. The data suggests that combined aPDT and antifungal therapy could be an alternative approach to antifungal-resistant Candida strains.
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionScience and Technology Planning Project of Guangdong Province, P.R. China
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionNIH
dc.descriptionUS Air Force MFEL Program
dc.descriptionUniv Estadual Paulista UNESP, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP, Brazil
dc.descriptionMassachusetts Gen Hosp, Div Infect Dis, Boston, MA 02114 USA
dc.descriptionFac Pindamonhangaba, Dept Restorat Dent, BR-12422970 Pindamonhangaba, SP, Brazil
dc.descriptionMassachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA
dc.descriptionNucl & Energy Res Inst, Ctr Lasers & Applicat, BR-05508000 Sao Paulo, Brazil
dc.descriptionSouthern Med Univ, Huiqiao Dept, Nanfang Hosp, Guangzhou 510515, Guangdong, Peoples R China
dc.descriptionSecond Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China
dc.descriptionUniv New Mexico, Dept Pathol, Albuquerque, NM 87131 USA
dc.descriptionUniv New Mexico, Ctr Mol Discovery, Albuquerque, NM 87131 USA
dc.descriptionHarvard Univ, Sch Med, Dept Dermatol, Boston, MA 02114 USA
dc.descriptionMIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
dc.descriptionBrown Univ, Warren Alpert Med Sch, Rhode Isl Hosp, Providence, RI 02903 USA
dc.descriptionBrown Univ, Warren Alpert Med Sch, Miriam Hosp, Providence, RI 02903 USA
dc.descriptionUniv Estadual Paulista UNESP, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP, Brazil
dc.descriptionCAPES: PDEE 2507-11-0
dc.descriptionScience and Technology Planning Project of Guangdong Province, P.R. China2011B080701091
dc.descriptionFAPESP: 12/19915-6
dc.descriptionNIHRO1 AI050875
dc.descriptionNIH5U54MH084690-02
dc.descriptionUS Air Force MFEL ProgramFA9550-04-1-0079
dc.format9
dc.languageeng
dc.publisherBiomed Central Ltd.
dc.relationBMC Microbiology
dc.relation2.829
dc.relation1,242
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCandida albicans
dc.subjectPhotodynamic therapy
dc.subjectGalleria mellonella
dc.titleSelective photoinactivation of Candida albicans in the non-vertebrate host infection model Galleria mellonella
dc.typeArtigo


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