dc.contributor | Univ Vale Paraiba | |
dc.contributor | Universidade Federal de Ouro Preto (UFOP) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.creator | Sakane, K. K. | |
dc.creator | Monteiro, C. J. | |
dc.creator | Silva, W. | |
dc.creator | Silva, A. R. | |
dc.creator | Santos, P. M. | |
dc.creator | Lima, K. F. | |
dc.creator | Moraes, K. C. M. [UNESP] | |
dc.date | 2014-12-03T13:10:59Z | |
dc.date | 2014-12-03T13:10:59Z | |
dc.date | 2014-01-01 | |
dc.date.accessioned | 2023-09-09T10:03:24Z | |
dc.date.available | 2023-09-09T10:03:24Z | |
dc.identifier | http://dx.doi.org/10.1590/1414-431X20133028 | |
dc.identifier | Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 47, n. 1, p. 50-59, 2014. | |
dc.identifier | 0100-879X | |
dc.identifier | http://hdl.handle.net/11449/112703 | |
dc.identifier | 10.1590/1414-431X20133028 | |
dc.identifier | S0100-879X2013005003028 | |
dc.identifier | WOS:000331907500007 | |
dc.identifier | S0100-879X2013005003028.pdf | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8762327 | |
dc.description | Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 mu M celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival. | |
dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description | Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) | |
dc.description | Fundacao Valeparaibana de Ensino | |
dc.description | Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, Brazil | |
dc.description | Univ Fed Ouro Preto, Nucleo Pesquisa Ciencias Biol, Ouro Preto, MG, Brazil | |
dc.description | Univ Estadual Paulista, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil | |
dc.description | Univ Estadual Paulista, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil | |
dc.description | CNPq: 475586/2009-3 | |
dc.description | CNPq: 506991/2010-5 | |
dc.description | FAPEMIG: 02351-10 | |
dc.format | 50-59 | |
dc.language | eng | |
dc.publisher | Associação Brasileira de Divulgação Científica (ABRADIC) | |
dc.relation | Brazilian Journal of Medical and Biological Research | |
dc.relation | 1.492 | |
dc.rights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | Cellular and molecular analyses | |
dc.subject | Cell death | |
dc.subject | Cyclooxygenase-2 inhibitor | |
dc.subject | H9c2 cardiac cell line | |
dc.title | Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms | |
dc.type | Artigo | |