Amperometric glucose biosensor based on layer-by-layer films of microperoxidase-11 and liposome-encapsulated glucose oxidase

dc.contributorUniversidade Federal de São Carlos (UFSCar)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.creatorGraca, J. S.
dc.creatorOliveira, R. F. de [UNESP]
dc.creatorMoraes, M. L. de
dc.creatorFerreira, M.
dc.date2014-12-03T13:08:52Z
dc.date2014-12-03T13:08:52Z
dc.date2014-04-01
dc.date.accessioned2023-09-09T09:46:55Z
dc.date.available2023-09-09T09:46:55Z
dc.identifierhttp://dx.doi.org/10.1016/j.bioelechem.2014.01.001
dc.identifierBioelectrochemistry. Lausanne: Elsevier Science Sa, v. 96, p. 37-42, 2014.
dc.identifier1567-5394
dc.identifierhttp://hdl.handle.net/11449/111651
dc.identifier10.1016/j.bioelechem.2014.01.001
dc.identifierWOS:000332191600005
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8761311
dc.descriptionAn important step in several bioanalytical applications is the immobilization of biomolecules. Accordingly, this procedure must be carefully chosen to preserve their biological structure and fully explore their properties. For this purpose, we combined the versatility of the layer-by-layer (LbL) method for the immobilization of biomolecules with the protective behavior of liposome-encapsulated systems to fabricate a novel amperometric glucose biosensor. To obtain the biosensing unit, an LbL film of the H2O2 catalyst polypeptide microperoxidase-11 (MP-11) was assembled onto an indium-tin oxide (ITO) electrode followed by the deposition of a liposome-encapsulated glucose oxidase (GOx) layer. The biosensor response toward glucose detection showed a sensitivity of 0.91 +/- 0.09 (mu A/cm(2))/mM and a limit of detection (LOD) of 8.6 +/- 1.1 mu M, demonstrating an improved performance compared to similar biosensors with a single phospholipid-liposome or even containing a non-encapsulated GOx layer. Finally, glucose detection was also performed in a zero-lactose milk sample to demonstrate the potential of the biosensor for food analysis. (C) 2014 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionnBioNet network (Brazil)
dc.descriptionUniv Fed Sao Carlos, UFSCar, BR-18052780 Sorocaba, SP, Brazil
dc.descriptionSao Paulo State Univ, UNESP, Postgrad Program Mat Sci & Technol, BR-17033360 Bauru, SP, Brazil
dc.descriptionUniv Fed Sao Paulo, UNIFESP, BR-12231280 Sao Jose Dos Campos, SP, Brazil
dc.descriptionSao Paulo State Univ, UNESP, Postgrad Program Mat Sci & Technol, BR-17033360 Bauru, SP, Brazil
dc.format37-42
dc.languageeng
dc.publisherElsevier B.V.
dc.relationBioelectrochemistry
dc.relation3.789
dc.relation0,850
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectGlucose biosensor
dc.subjectMicroperoxidase-11
dc.subjectLayer-by-layer technique
dc.subjectLiposome
dc.subjectBiosensor
dc.titleAmperometric glucose biosensor based on layer-by-layer films of microperoxidase-11 and liposome-encapsulated glucose oxidase
dc.typeArtigo


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