The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.creatorOliveira, Sarah Almeida Coelho
dc.creatorZambrana, Jéssica Rabelo Mina
dc.creatorDi Iorio, Fernanda Bispo Reis
dc.creatorPereira, Cristiane Aparecida
dc.creatorJorge, Antonio Olavo Cardoso [UNESP]
dc.date2014-09-30T18:18:33Z
dc.date2014-09-30T18:18:33Z
dc.date2014-01-01
dc.date.accessioned2023-09-09T09:10:44Z
dc.date.available2023-09-09T09:10:44Z
dc.identifierhttp://dx.doi.org/10.1590/S1806-83242013005000024
dc.identifierBrazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 28, n. 1, p. 22-27, 2014.
dc.identifier1806-8324
dc.identifierhttp://hdl.handle.net/11449/109663
dc.identifier10.1590/S1806-83242013005000024
dc.identifierS1806-83242014000100217
dc.identifierWOS:000328723700004
dc.identifierS1806-83242014000100217.pdf
dc.identifier0053567153623569
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8759380
dc.descriptionThe aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases.
dc.descriptionUniversidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral Diagnosis
dc.descriptionUniversidade Federal de São Paulo Institute of Environmental, Chemical and Pharmaceutics Sciences Department of Earth and Exact Sciences
dc.descriptionUniversidade Estadual Paulista Institute of Science and Technology Department of Biosciences and Oral Diagnosis
dc.format22-27
dc.languageeng
dc.publisherSociedade Brasileira de Pesquisa Odontológica (SBPqO)
dc.relationBrazilian Oral Research
dc.rightsAcesso aberto
dc.sourceSciELO
dc.subjectBiofilms
dc.subjectCitrus limonum
dc.subjectCitrus aurantium
dc.titleThe antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms
dc.typeArtigo


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