Opposite effects of bFGF and TGF-beta on collagen metabolism by human periodontal ligament fibroblasts

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGonzales Silverio-Ruiz, Karina
dc.creatorTraverso Martinez, Aurora Esmeralda
dc.creatorPompermaier Garlet, Gustavo
dc.creatorFregonesi Barbosa, Carolina
dc.creatorSantana Silva, Joao
dc.creatorBarreto Cicarelli, Regina Maria
dc.creatorValentini, Sandro Roberto
dc.creatorGeorges Abi-Rached, Ricardo Sarnih
dc.creatorRossa, Carlos Junior
dc.date2014-05-20T15:19:51Z
dc.date2016-10-25T17:52:47Z
dc.date2014-05-20T15:19:51Z
dc.date2016-10-25T17:52:47Z
dc.date2007-08-01
dc.date.accessioned2017-04-05T23:22:25Z
dc.date.available2017-04-05T23:22:25Z
dc.identifierCytokine. London: Academic Press Ltd Elsevier B.V. Ltd, v. 39, n. 2, p. 130-137, 2007.
dc.identifier1043-4666
dc.identifierhttp://hdl.handle.net/11449/31237
dc.identifierhttp://acervodigital.unesp.br/handle/11449/31237
dc.identifier10.1016/j.cyto.2007.06.009
dc.identifierWOS:000250913700006
dc.identifierhttp://dx.doi.org/10.1016/j.cyto.2007.06.009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/875825
dc.descriptionThis study evaluated the effects of bFGF and TGF-beta, individually and combined, on cell proliferation and collagen metabolism. Primary human periodontal ligament cells were stimulated with two concentrations (I and 10 ng/ml) of each growth factor, both individually and combined. Proliferation was determined by a commercial biochemical assay. Real time RT-PCR determined gene expression of NMP-1 and -2, collagen types I and III, TIMP-1, -2 and -3. Autocrine effects on synthesis of bFGF and TGF-beta were evaluated by ELISA. Only TGF-beta, either isolated or associated with bFGF, significantly increased cell proliferation. TGF-beta had anabolic effects, increasing expression of type I and III collagen as well as of TIMPs, whereas bFGF had opposite effects. When bFGF and TGF-beta were associated, the anabolic effects prevailed. Synthesis of TGF-beta was induced only by the association of lower concentrations of the growth factors, whereas there was a dose-dependent production of bFGF. It is concluded that bFGF had a predominantly catabolic effect, and TGF-beta exerted an anabolic effect on hPDL cells. (c) 2007 Elsevier Ltd. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationCytokine
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecthuman periodontal ligament cells
dc.subjecttransforming growth factor beta
dc.subjectbasic fibroblast growth factor
dc.subjectcollagen
dc.subjectmatrix metalloproteases
dc.subjecttissue inhibitor of matrix metalloproteases
dc.subjectperiodontal regeneration
dc.titleOpposite effects of bFGF and TGF-beta on collagen metabolism by human periodontal ligament fibroblasts
dc.typeOtro


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