Validación de la interacción entre el efector XopAE de Xanthomonas phaseoli pv. manihotis y una patelina de plantas involucrada en inmunidad vegetal

dc.contributorBernal Giraldo, Adriana Jimena
dc.contributorTrujillo Beltrán, César Augusto
dc.contributorBejarano Franco, Daniela
dc.contributorGaravito Aguilar, Zayra Viviana
dc.contributorLópez Carascal, Camilo Ernesto
dc.contributorLaboratorio de Interacciones Moleculares de Microorganismos Agrícolas (LIMMA)
dc.creatorRodríguez Mantilla, Carlos José
dc.date.accessioned2028-11-16
dc.date.accessioned2023-09-07T02:09:33Z
dc.date.available2028-11-16
dc.date.available2023-09-07T02:09:33Z
dc.date.created2028-11-16
dc.date.issued2023-06-06
dc.identifierhttp://hdl.handle.net/1992/69008
dc.identifierinstname:Universidad de los Andes
dc.identifierreponame:Repositorio Institucional Séneca
dc.identifierrepourl:https://repositorio.uniandes.edu.co/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8729006
dc.description.abstractEl añublo bacteriano de la yuca (CBB, por sus siglas en inglés), causado por Xanthomonas phaseoli pv. manihotis (Xpm), es la enfermedad bacteriana más importante de la yuca y afecta la producción de este cultivo en varios países tropicales en todo el mundo. Al igual que otras Xanthomonas fitopatógenas, Xpm requiere efectores tipo III (T3E) para su plena virulencia, colonización bacteriana y el desarrollo exitoso de la CBB. XopAE, un efector de la familia de proteínas externas de Xanthomonas (Xop), constituye uno de los T3E más conservados en Xpm. Este trabajo tuvo como objetivo evaluar la interacción molecular entre XopAEXpm y las patelinas MePATL3 y AtPATL3. Nuestro análisis bioinformático sugiere que MePATL3 es una proteína de tipo Sec14-GOLD PITP, y que presenta homología estructural con AtPATL3 basado en la identificación de dominios CRAL/TRIO y GOLD altamente conservados. Además, realizamos ensayos de doble híbrido de levadura (Y2H, por sus siglas en inglés) para evaluar la interacción entre XopAEXpm y MePATL3 en su extensión completa, así como entre XopAEXpm y versiones truncadas de AtPATL3, lo que nos permitió identificar la región N-terminal y/o el dominio C-terminal CRAL/TRIO de AtPATL3 como puntos críticos de interacción con XopAEXpm. Por último, realizamos ensayos de Western Blot expresando MePATL3 en N. benthamiana como un primer paso para los ensayos de Co-Inmunoprecipitación in planta.
dc.languagespa
dc.publisherUniversidad de los Andes
dc.publisherMaestría en Ciencias Biológicas
dc.publisherFacultad de Ciencias
dc.publisherDepartamento de Ciencias Biológicas
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dc.rightsinfo:eu-repo/semantics/openAccess
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dc.titleValidación de la interacción entre el efector XopAE de Xanthomonas phaseoli pv. manihotis y una patelina de plantas involucrada en inmunidad vegetal
dc.typeTrabajo de grado - Maestría


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