Rol del T6SS en Competencia Bacteriana de Xanthomonas phaseoli pv. manihotis Ante Bacterias Epífitas de Yuca (Manihot esculenta)

dc.contributorBernal Giraldo, Adriana Jimena
dc.contributorZambrano Eder, María Mercedes
dc.contributorVillegas Torres, María Francisca
dc.contributorLaboratorio de Interacciones Moleculares de Microorganismos en Agricultura - LIMMA
dc.creatorHernández, Erick Geovanni
dc.date.accessioned2023-02-06T18:57:58Z
dc.date.accessioned2023-09-07T01:18:55Z
dc.date.available2023-02-06T18:57:58Z
dc.date.available2023-09-07T01:18:55Z
dc.date.created2023-02-06T18:57:58Z
dc.date.issued2023-02-05
dc.identifierhttp://hdl.handle.net/1992/64721
dc.identifierinstname:Universidad de los Andes
dc.identifierreponame:Repositorio Institucional Séneca
dc.identifierrepourl:https://repositorio.uniandes.edu.co/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8728259
dc.description.abstractMediante el Sistema de secreción tipo VI (T6SS), las bacterias gramnegativas pueden translocar proteínas efectoras que juegan un papel importante en competencia bacteriana y virulencia hacia hospederos eucariotas. En este estudio, fue posible realizar el aislamiento e identificación taxonómica mediante secuenciación del gen 16S, de seis bacterias epífitas que habitan la filósfera de yuca (Manihot esculenta), una especie vegetal de importancia económica en Sur América y África. Ensayos de competencia bacteriana in vitro sugirieron que el T6SS de la bacteria fitopatógena Xanthomonas phaseoli pv. manihotis (Xpm) era requerido por contacto directo para ejercer competencia bacteriana hacia la bacteria epífita de yuca Rhodococcus sp. Mutantes para los genes VgrG, ClpV y Hcp del T6SS de Xpm fueron incapaces de inhibir el crecimiento de Rhodococcus sp. Sin embargo, no fue posible determinar con certeza que el T6SS de Xpm sea el responsable de la inhibición de Rhodococcus sp., debido a la falta de complementación observada de estos mutantes en Xpm. Finalmente, fue posible predecir a través de análisis bioinformáticos dos proteínas efectoras que serían hipotéticamente traslocadas por el T6SS de Xpm, y el papel que podrían cumplir en la competencia bacteriana. Los resultados obtenidos en este estudio ayudan a comprender de alguna manera, cómo el T6SS podría jugar un papel importante en competencia bacteriana posibilitando que Xpm gane ventaja de nicho al matar a otras bacterias, facilitando finalmente el establecimiento de la enfermedad tizón bacteriano de la yuca (CBB).
dc.description.abstractThrough the Type VI Secretion System (T6SS), Gram-negative bacteria can translocate effector proteins that play an important role in bacterial competition and virulence towards eukaryotic hosts. In this study, it was possible to perform the isolation and taxonomic identification through 16S gene sequencing of six epiphytic bacteria that inhabit the cassava (Manihot esculenta) phyllosphere, a plant species of economic importance in South America and Africa. In vitro bacterial competition assays suggested that T6SS from the phytopathogenic bacterium Xanthomonas phaseoli pv. manihotis (Xpm) was required by direct contact to exert bacterial competition towards the cassava epiphytic bacterium Rhodococcus sp. Mutants for the VgrG, ClpV and Hcp genes of the Xpm T6SS were unable to inhibit the growth of Rhodococcus sp. However, it was not possible to determine with certainty that the T6SS of Xpm is responsible for the inhibition of Rhodococcus sp., due to the observed lack of complementation of these mutants in Xpm. Finally, it was possible to predict through bioinformatic analysis two effector proteins that would be hypothetically translocated by the T6SS of Xpm, and the role that they could play in bacterial competition. The results obtained in this study help to understand in some way, how T6SS could play an important role in bacterial competition, enabling Xpm to gain a niche advantage by killing other bacteria, finally facilitating the establishment of cassava bacterial blight disease (CBB).
dc.languagespa
dc.publisherUniversidad de los Andes
dc.publisherMaestría en Ciencias Biológicas
dc.publisherFacultad de Ciencias
dc.publisherDepartamento de Ciencias Biológicas
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dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.titleRol del T6SS en Competencia Bacteriana de Xanthomonas phaseoli pv. manihotis Ante Bacterias Epífitas de Yuca (Manihot esculenta)
dc.typeTrabajo de grado - Maestría


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