Evaluación del uso de bacteriófagos para el biocontrol de aislamientos de Klebsiella pneumoniae multidrogoresistente en cuatro materiales de superficies comunes en entornos clínicos

dc.contributorVives Flórez, Martha Josefina
dc.contributorCelis Ramírez, Adriana Marcela
dc.contributorValderrama Aguirre, Augusto Elías
dc.contributorJiménez Quiceno, Judy Natalia
dc.contributorCIMIC
dc.creatorÁlvarez Osorio, Andrea Katherine
dc.date.accessioned2023-08-08T20:20:52Z
dc.date.accessioned2023-09-07T00:17:19Z
dc.date.available2023-08-08T20:20:52Z
dc.date.available2023-09-07T00:17:19Z
dc.date.created2023-08-08T20:20:52Z
dc.date.issued2023-06-06
dc.identifierhttp://hdl.handle.net/1992/69440
dc.identifierinstname:Universidad de los Andes
dc.identifierreponame:Repositorio Institucional Séneca
dc.identifierrepourl:https://repositorio.uniandes.edu.co/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8727266
dc.description.abstractLa propagación de Infecciones Asociadas a la Atención en Salud (IAAS), principalmente por contaminación bacteriana, representa una seria amenaza para los sistemas de salud. La prevalencia de K. pneumoniae en hisopos de superficie, su detección en distintos materiales, la creciente resistencia global a carbapenémicos con tasas de mortalidad de 22-72% y cepas resistentes a desinfectantes, acentúan la necesidad de opciones biotecnológicas. Con el fin de explorar una alternativa de descontaminación efectiva y específica para el control de aislamientos de K. pneumoniae multirresistente, este estudio investigó el potencial de los fagos para controlar la carga bacteriana en materiales de superficies comunes en entornos clínicos: acero, tela, PVC y poliestireno. Se determinó que los aislamientos de K. pneumoniae sobrevivieron hasta 24 horas en superficies, con reducciones entre 0.49-1.59 y 1.69-3.88 LOG UFC a 24°C y 37°C, respectivamente. Posteriormente, se seleccionaron tres bacteriófagos de aguas residuales hospitalarias, que en conjunto mostraron actividad lítica contra el 88% de los aislamientos hospitalarios, además, de actividad antimicrobiana en superficies, logrando reducciones de hasta 4.99 LOG UFC. Estos resultados respaldan la efectividad de los fagos como desinfectantes y sugieren su potencial uso complementario en situaciones de brote en entornos hospitalarios.
dc.languagespa
dc.publisherUniversidad de los Andes
dc.publisherMaestría en Ciencias Biológicas
dc.publisherFacultad de Ciencias
dc.publisherDepartamento de Ciencias Biológicas
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dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.titleEvaluación del uso de bacteriófagos para el biocontrol de aislamientos de Klebsiella pneumoniae multidrogoresistente en cuatro materiales de superficies comunes en entornos clínicos
dc.typeTrabajo de grado - Maestría


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