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Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
Registro en:
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 1, p. 59-61, 2007.
0074-0276
10.1590/S0074-02762007000100009
S0074-02762007000100009
S0074-02762007000100009.pdf
Autor
Cardoso, Rosilene Fressatti
Cardoso, Marco Antonio
Leite, Clarice Queico Fujimura
Sato, Daisy Nakamura
Mamizuka, Elsa Masae
Hirata, Rosário Dominguez Crespo
Mello, Fernando Fiúza de
Hirata, Mario Hiroyuki
Resumen
Resistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10%) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.