Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMorais, Glaucia Almeida de
dc.creatorTakaki, Massanori
dc.date2014-05-20T15:07:27Z
dc.date2016-10-25T17:42:14Z
dc.date2014-05-20T15:07:27Z
dc.date2016-10-25T17:42:14Z
dc.date1998-06-01
dc.date.accessioned2017-04-05T22:37:16Z
dc.date.available2017-04-05T22:37:16Z
dc.identifierBrazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 41, n. 1, p. 17-25, 1998.
dc.identifier1516-8913
dc.identifierhttp://hdl.handle.net/11449/26518
dc.identifierhttp://acervodigital.unesp.br/handle/11449/26518
dc.identifier10.1590/S1516-89131998000100003
dc.identifierS1516-89131998000100003
dc.identifierS1516-89131998000100003.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1516-89131998000100003
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/871224
dc.descriptionDetermination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
dc.languageeng
dc.publisherBrazilian Archives of Biology and Technology
dc.relationBrazilian Archives of Biology and Technology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectdetermination
dc.subjectselective inactivation
dc.subjectPhaseolus vulgaris
dc.titleDetermination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
dc.typeOtro


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