Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorResende, Flavia Aparecida
dc.creatorVilegas, Wagner
dc.creatorSantos, Lourdes Campaner dos
dc.creatorVaranda, Eliana Aparecida
dc.date2014-05-20T14:21:08Z
dc.date2016-10-25T17:41:52Z
dc.date2014-05-20T14:21:08Z
dc.date2016-10-25T17:41:52Z
dc.date2012-05-01
dc.date.accessioned2017-04-05T22:35:31Z
dc.date.available2017-04-05T22:35:31Z
dc.identifierMolecules. Basel: Mdpi Ag, v. 17, n. 5, p. 5255-5268, 2012.
dc.identifier1420-3049
dc.identifierhttp://hdl.handle.net/11449/26323
dc.identifierhttp://acervodigital.unesp.br/handle/11449/26323
dc.identifier10.3390/molecules17055255
dc.identifierWOS:000304587600040
dc.identifierWOS000304587600040.pdf
dc.identifierhttp://dx.doi.org/10.3390/molecules17055255
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/871064
dc.descriptionThe mutagenicity of ten flavonoids was assayed by the Ames test, in Salmonella typhimurium strains TA98, TA100 and TA102, with the aim of establishing hydroxylation pattern-mutagenicity relationship profiles. The compounds assessed were: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone. In the Ames assay, quercetin acted directly and its mutagenicity increased with metabolic activation. In the presence of S9 mix, kaempferol and galangin were mutagenic in the TA98 strain and kaempferol showed signs of mutagenicity in the other strains. The absence of hydroxyl groups, as in flavone, only signs of mutagenicity were shown in strain TA102, after metabolization and, among monohydroxylated flavones (3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone), the presence of hydroxyl groups only resulted in minor changes. Luteolin and fisetin also showed signs of mutagenicity in strain TA102. Finally, chrysin, which has only two hydroxy groups, at the 5-OH and 7-OH positions, also did not induce mutagenic activity in any of the bacterial strains used, under either activation condition. All the flavonoids were tested at concentrations varying from 2.6 to 30.7 nmol/plate for galangin and 12.1 to 225.0 nmol/plate for other flavonoids. In light of the above, it is necessary to clarify the conditions and the mechanisms that mediate the biological effects of flavonoids before treating them as therapeutical agents, since some compounds can be biotransformed into more genotoxic products; as is the case for galangin, kaempferol and quercetin.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherMdpi Ag
dc.relationMolecules
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmutagenicity
dc.subjectAmes test
dc.subjectflavonoids
dc.titleMutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test
dc.typeOtro


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